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Plasmid#222823PurposeBacteria expression of His-GST -ATG13 C-terminalDepositorInsertATG13 (ATG13 Human)
UseTags6His-GSTExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1477
Plasmid#213803PurposeBsaI-associated switch methylase subunit SDepositorInsertBsaI-associated switch methylase S.Sen0738I
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1475
Plasmid#213801PurposeBsaI-associated switch methylase subunit MDepositorInsertBsaI-associated switch methylase M.Sen0738I
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
STREP-SUMO-FLAG-SeKaiC
Plasmid#218179PurposeWT SeKaiC for phosphorylation/dephosphorylation studyDepositorInsertSTREP-SUMO-FLAG-SeKaiC
UseTagsSTREP-TagExpressionBacterialMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGS Q16C
Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS8
Plasmid#218716PurposeExpresses N-terminally EGFP-tagged BBS8 in mammalian cellsDepositorInsertBBS8 (TTC8 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT70B
Plasmid#218729PurposeExpresses N-terminally EGFP-tagged IFT70B in mammalian cellsDepositorInsertIFT70B (TTC30B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL038 Lenti U6 dCas12a gRNA Puro mCherry
Plasmid#195546PurposedCas12a gRNA expression backboneDepositorArticleInsertLenti U6- empty cassette_Direct repeat_puro_mcherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT314 AAV-Rapamycin Inducible-NZ-Citrine
Plasmid#202063PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-NZ-Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT365 AAV-Rapamycin Inducible-S3H
Plasmid#202066PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-S3H
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT366 AAV-Rapamycin Inducible QNZF
Plasmid#202067PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible QNZF
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT310 AAV-BFPdonor
Plasmid#202057PurposeAAV HDR donorDepositorArticleInsertAAV-BFPdonor
UseAAV; Aav packaging plasmidTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT313 AAV-Rapamycin Inducible-ZF-Citrine
Plasmid#202062PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-ZF-Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Key
Plasmid#216503PurposeRas activity dependent proximity labeler, Key only, untargetedDepositorInsertRas-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Cage
Plasmid#216502PurposeRas activity dependent proximity labeler, Cage only, untargetedDepositorInsertRas-LOCKR-PL Cage
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-S R89L (negative control)
Plasmid#216497PurposeFRET-based reporter for Ras activity, untargeted, negative controlDepositorInsertRas-LOCKR-S R89L negative control
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL635
Plasmid#189829PurposeThe T-DNA backbone of Cas12j2 for genome editing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna cloneTagsccdbExpressionMutationPromoterZmUbi1Available sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL637
Plasmid#189832PurposeThe T-DNA backbone of vCas12j2 for genome editing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna cloneTagsExpressionMutationPromoterZmUbi1Available sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL639
Plasmid#189833PurposeThe T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna cloneTagsExpressionMutationPromoterZmUbi1Available sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
MP6-SlugABEN-738
Plasmid#214348PurposeExpresses N-terminus of ABE, and untargeted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only