Addgene

We narrowed to 13,597 results for: pET

Showing: 6301 - 6320 of 13597 results

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pLJM1-EGFP

Plasmid

#19319

Purpose

3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagD shRNA #1

Plasmid

#30321

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1 RagD shRNA #2

Plasmid

#30322

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
paavCAG-post-mGRASP-2A-dTomato

Plasmid

#34912

Purpose

post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

postsynaptic mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 121L

Plasmid

#19309

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q121L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-HA GST RagB 99L

Plasmid

#19303

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q99L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 77L

Plasmid

#19308

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

S77L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pWS5397-CRISPRai-NatR

Plasmid

#182836

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGH8 - pRAB-3::mCherry::unc-54utr

Plasmid

#19359

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Prab-3::mCherry

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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MS2_GFP

Plasmid

#61764

Purpose

Expresses MS2 coat protein fused to GFP

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

MS2 coat protein fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Ubc promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pFZ19

Plasmid

#36184

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Tags

Expression

Plant

Mutation

Promoter

XVE

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-mCerulean

Plasmid

#34910

Purpose

pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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Cyto-V5-APEX2

Plasmid

#170572

Purpose

Expresses APEX2 in cytosol

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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MLM3613

Plasmid

#42251

Purpose

Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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