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Plasmid#224409PurposeMammalian expression of KIF 13a S1371ADepositorInsertKIF 13a S1371A (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationS1371APromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AWLDLE
Plasmid#224410PurposeMammalian expression of KIF 13a WLDLEDepositorInsertKIF 13a WLDLE (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationWLDLEPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.M6PR-GFP
Plasmid#224407PurposeMammalian expression of M6PRDepositorInsertM6PR (M6PR Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA1
Plasmid#215398PurposeCm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)DepositorInsertcspA1
UseTags6xHis TagExpressionBacterialMutationCodon-optimized for E. coli BL21(DE3)PromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Spo20(PABD)
Plasmid#221758PurposeStable expression of GFP-Spo20(PABD) in mammalian cellsDepositorInsertSpo20 (SOS1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem Spo20(PABD) (amino acids 422-551)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TAPP1(PHD)
Plasmid#221757PurposeStable expression of GFP-TAPP1(PHD) in mammalian cellsDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TAPP1 (PHD) (amino acids 184-304)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PKD C1ab
Plasmid#221755PurposeStable expression of GFP-PKD C1ab in mammalian cellsDepositorInsertPrkd1(138-343) (Prkd1 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 138-343PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA4114_Cterm_His
Plasmid#220484Purposeheterologous expression of PA4114 for protein purificationDepositorInsertPA4114
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA1472_Cterm_His
Plasmid#220485Purposeheterologous expression of PA1472 for protein purificationDepositorInsertPA1472
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria_speG_M15
Plasmid#220488Purposeinducible CRISPRiDepositorInsertgspeG
UseCRISPR and RNAiTagsExpressionMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria_speG_E23
Plasmid#220489Purposeinducible CRISPRiDepositorInsertgspeG
UseCRISPR and RNAiTagsExpressionMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speG_genomic
Plasmid#220473Purposeheterologous expression under endogenous speG E. coli promoterDepositorInsertspeG
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA4114
Plasmid#220474Purposeheterologous expression of PA4114 under endogenous speG E. coli promoterDepositorInsertPA4114
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1472
Plasmid#220475Purposeheterologous expression of PA1472 under endogenous speG E. coli promoterDepositorInsertPA1472
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1377
Plasmid#220476Purposeheterologous expression of PA1377 under endogenous speG E. coli promoterDepositorInsertPA1377
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_speG_Nterm_His
Plasmid#220478Purposeheterologous expression for protein purificationDepositorInsertspeG
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA4114_Nterm_His
Plasmid#220479Purposeheterologous expression of PA4114 for protein purificationDepositorInsertPA4114
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA1472_Nterm_His
Plasmid#220480Purposeheterologous expression of PA1472 for protein purificationDepositorInsertPA1472
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only