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Plasmid#132937PurposeAll-in-one huTRIM5 rescue-control shRNA knockdownDepositorInserthuman TRIM5
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD38-52
Plasmid#98685PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 38-52, residues 1853-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUPI
Plasmid#80279PurposeA bicistronic lentiviral vector used to stably express cDNAs.DepositorInsertPuro resistance cassette, IRES, cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-37
Plasmid#98686PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 27-37, residues 1773-1852PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUPI AoT5Cyp
Plasmid#80280PurposeExpresses Aotus TRIM5 CypA fusionDepositorInsertTRIM5 CypA fusion
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK025
Plasmid#105415PurposeSynthetic biologyDepositorInsertHormone-binding domain and regulatory sequences (estrogen receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-N1-PDZ1-GAGA
Plasmid#31651DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsHis, S-tagExpressionMammalianMutationchanged P(21)NGYGF changed to H(21)NGAGAPromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-N1-PDZ2-GAGA
Plasmid#32857DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsHis,S-tagExpressionMammalianMutationPDZ2 P(161)NGYGF mutated to H(161)NGAGAPromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD43-52
Plasmid#98684PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationhepatds 43-52, residues 1888-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)_G
Plasmid#146402PurposeBacterial Expression of DmDCP1_328-366DepositorInsertDmDCP1_328-366 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTEI037
Plasmid#105413PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor) fused to mEGFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI041
Plasmid#105414PurposeSynthetic biologyDepositorInsertHormone-binding domain and regulatory sequences (estrogen receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_S1966C
Plasmid#98681PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationS1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM9,10
Plasmid#170280PurposeExpresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertUseTagsExpressionBacterialMutationPromoterTim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…Available sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI-D10R-E48R
UseTagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
mitoTALECD for_-20G_1333CN
Plasmid#186200PurposeFor genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87DepositorInsertsmitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
UseSynthetic Biology; Ti plasmid for plant transform…TagsExpressionMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoTALECD for_ -13G_1397CN
Plasmid#186199PurposeFor genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87DepositorInsertsmitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
UseSynthetic Biology; Ti plasmid for plant transform…TagsExpressionMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoTALECD for_ -6G_1333CN
Plasmid#186198PurposeFor genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87DepositorInsertsmitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
UseSynthetic Biology; Ti plasmid for plant transform…TagsExpressionMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPI-mPAX4
Plasmid#114306PurposeConstitutive expression of the Pax4 trancription factorDepositorInsertPAX4 (Pax4 Mouse)
UseLentiviralTagsc-myc epitope, 6xHis tagExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only