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Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5419_p3WJdB-glcO36
Plasmid#221193PurposeGlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminatorDepositorInsertT7 promoter-glcO36
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO36 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSDM052
Plasmid#194341PurposepUC mini-Tn7 vector expressing LseCas13aDepositorInsertCas13a(Lse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-inclusion-GLuc
Plasmid#218671PurposeAlternative splicing signaling: signal increased with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP-SNX9
Plasmid#170551PurposeTo monitor the turnover of SNX9 in lysosomesDepositorInsertSNX9 (SNX9 Human)
UseRetroviralTagsSNX9 in tandem with mCherry and GFPExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-Nm2Cas9-BlastR
Plasmid#220980PurposeExpresses human codon-optimized Nm2Cas9DepositorInsertNm2Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-CjCas9-BlastR
Plasmid#220979PurposeExpresses human codon-optimized CjCas9DepositorInsertCjCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-enCjCas9-BlastR
Plasmid#220978PurposeExpresses human codon-optimized enCjCas9DepositorInsertenCjCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-St1Cas9-BlastR
Plasmid#220976PurposeExpresses human codon-optimized St1Cas9DepositorInsertSt1Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SlugCas9-HF-BlastR
Plasmid#220973PurposeExpresses human codon-optimized SlugCas9-HFDepositorInsertSlugCas9-HF
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_VPS35L(A830T)
Plasmid#209693PurposeExpresses MAC3-tagged VPS35L(A830T) in mammalian cellsDepositorInsertVPS35L (VPS35L Human)
UseTagsMAC3-tagExpressionMammalianMutationA830T, changed Alanine 830 to ThreoninePromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SauriCas9-KKH-BlastR
Plasmid#220972PurposeExpresses human codon-optimized SauriCas9-KKHDepositorInsertSauriCas9-KKH
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-efSaCas9-BlastR
Plasmid#220971PurposeExpresses human codon-optimized efSaCas9DepositorInsertefSaCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-Sa-SlugCas9-BlastR
Plasmid#220968PurposeExpresses human codon-optimized Sa-SlugCas9DepositorInsertSa-SlugCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SauriCas9-BlastR
Plasmid#220967PurposeExpresses human codon-optimized SauriCas9DepositorInsertSauriCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SlugCas9-BlastR
Plasmid#220965PurposeExpresses human codon-optimized SlugCas9DepositorInsertSlugCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-sRGN3.1-BlastR
Plasmid#220964PurposeExpresses human codon-optimized sRGN3.1DepositorInsertsRGN3.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1478
Plasmid#213804PurposeBsaI-associated switch methylase subunit SDepositorInsertBsaI-associated switch methylase S.Sen0738I
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1476
Plasmid#213802PurposeBsaI-associated switch methylase subunit MDepositorInsertBsaI-associated switch methylase M.Sen0738I
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -