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Showing: 6281 - 6300 of 6547 results

  1. Flag-M4-AR-W741C-S81A-P82G

    Plasmid
    #171251
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)
    Depositor
    Steven Balk
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    171251-plasmid-map-sequence-id-335920

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  2. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid
    #120250
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120250-plasmid-map-sequence-id-238538

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  7. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid
    #119554
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    119554-plasmid-map-sequence-id-238815

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  8. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74630-plasmid-map-sequence-id-373032

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  9. SP6-VEE-GFP

    Plasmid
    #58976
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58976-plasmid-map-sequence-id-445055

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  11. pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

    Plasmid
    #70231
    Purpose
    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
    Depositor
    Steven Henikoff
    Article
    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.
    Insert
    3xFLAG tag and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70231-plasmid-map-sequence-id-129162

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  12. pICE-FLAG-NAT10-siR-WT

    Plasmid
    #59365
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    human NAT10 (NAT10 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Mutations to render cDNA resistant to a siRNA
    Promoter
    CMV-tet
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59365-plasmid-map-sequence-id-92043

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  14. pICE-FLAG-NAT10-siR-G641E

    Plasmid
    #59366
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    NAT10 NM_024662 (NAT10 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    G641E and silent mutations to render the cDNA res…
    Promoter
    CMV-tet
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59366-plasmid-map-sequence-id-92067

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  16. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172316
    Purpose
    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172316-plasmid-map-sequence-id-343650

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  17. pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)

    Plasmid
    #70232
    Purpose
    ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
    Depositor
    Steven Henikoff
    Article
    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.
    Insert
    3xFLAG tag and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70232-plasmid-map-sequence-id-129164

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Showing: 6281 - 6300 of 6547 results