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Plasmid#30104DepositorInsertpCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsT7noRBS, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
GUCY1B3
Plasmid#39121PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGUCY1B3 (GUCY1B1 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationThis is a complex of two proteins; GUCY1A3 (aa 46…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGS1044
Plasmid#63797PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection markerDepositorInsert3xFLAG followed by KlLEU2
UseE. coli cloning vectorTags3xFLAGExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKO-Paxillin-22
Plasmid#57849PurposeLocalization: Focal Contacts, Excitation: 548, Emission: 561DepositorInsertPaxillin
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
YCplac111-KOG1 (pAN58)
Plasmid#39209DepositorInsertKOG1 (KOG1 Budding Yeast)
UseTagsmycExpressionYeastMutationL1512PPromoterKOG1Available sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3152 (=HL 716 + pHL 1056)
Plasmid#30048DepositorInsertpCon:MicC, pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK c14orf28 3'UTR
Plasmid#19856DepositorInsertc14orf28 3'UTR (C14orf28 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
BacPAK Flag-NFRKB 1-465
Plasmid#19788DepositorInsertNFRKB (NFRKB Human)
UseTagsFlag and HisExpressionInsectMutationonly contains amino acids 1-465PromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
HL 2817 (=HL 716 + pHL 720)
Plasmid#30031DepositorInsertpLtetO-1:mCherry, pLlacO-1:sodB::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4120 (=HL 716 + pHL 1370)
Plasmid#30094DepositorInsertpLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4180 (=HL 2752 + pHL 1358)
Plasmid#30099DepositorInsertpLlacO-1:hfq, pLtetO-1m9:sodB::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR-fishRab8B[Q67L]
Plasmid#24908DepositorInsertRab8B (rab8b Zebrafish)
UseGateway entry vectorTagsExpressionMutationChanged Glutamine 67 to LeucinePromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
Dom34 K82A
Plasmid#19112DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationK82APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
SSO0083
Plasmid#19097DepositorInsertPelA (Cell division protein pelota homolog) (Orf c04039 protein)
UseTagsN tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5419_p3WJdB-glcO36
Plasmid#221193PurposeGlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminatorDepositorInsertT7 promoter-glcO36
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO36 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSDM052
Plasmid#194341PurposepUC mini-Tn7 vector expressing LseCas13aDepositorInsertCas13a(Lse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-inclusion-GLuc
Plasmid#218671PurposeAlternative splicing signaling: signal increased with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP-SNX9
Plasmid#170551PurposeTo monitor the turnover of SNX9 in lysosomesDepositorInsertSNX9 (SNX9 Human)
UseRetroviralTagsSNX9 in tandem with mCherry and GFPExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-Nm2Cas9-BlastR
Plasmid#220980PurposeExpresses human codon-optimized Nm2Cas9DepositorInsertNm2Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only