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Plasmid#44825DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationQ67L (constitutive active)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1L16A/L17A
Plasmid#13478DepositorInsertMKP-1L16A/L17A (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationChanged Leucine 16 to Alanine; changed Leucine 1…PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab5 DN
Plasmid#37694DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS43N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab5 CA
Plasmid#37702DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ88L (constitutive active)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-Venus-FLAG-LINXa4-Bre1
Plasmid#73618PurposeVisualizing the nuclear translocation of LINXa4-Bre1 in yeast.DepositorInsertVenus
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT A41AA C55A sfGFP 150TAG
Plasmid#154770Purposeplasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG in GFP reporter, G1 PylT with A41AA and C5…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pIK137
Plasmid#65632PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragmentDepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
UseTagsExpressionWormMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha D291N mutant GFP
Plasmid#128821PurposeTo visualize Doc2alpha D291N mutant in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationD291NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta D303N mutant GFP
Plasmid#128822PurposeTo visualize Doc2beta D303N mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationD303NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Grb10-WT
Plasmid#74501PurposeOverexpression of Grb10 in mammalian cellsDepositorInsertGrb10 (GRB10 Human)
UseTagsFLAGExpressionMammalianMutationL358S, V522A, and V524LPromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Sa sgRNA expression vector
Plasmid#107720PurposeFor in vitro transcription of Sa sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 DN
Plasmid#44826DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationT22N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Alpha-Actinin-19
Plasmid#57944PurposeLocalization: Alpha Actinin, Excitation: 549, Emission: 565DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
kank3_pJet1.2
Plasmid#121981Purposefor generating kank3 riboprobes for WISH in zebrafishDepositorInsertkank3 (kank3 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-7
Plasmid#126057PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab7 CA
Plasmid#37704DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab8 CA
Plasmid#37707DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only