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Plasmid#68163PurposeProvides the CaMV 35S Promoter as a level 0 GoldenBraid partDepositorInsertCaMV 35S promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2R
Plasmid#68231PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 L171C
Plasmid#66177PurposeExpresses the L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationL171CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C
Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR
Plasmid#61778PurposeLuciferase reporter containing the 3'UTR of mouse YwhagDepositorInsertmouse Ywhag 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 4175 (=HL 716 + pHL 1391)
Plasmid#30096DepositorInsertpLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 1329 (=HL 865 + pHL 344)
Plasmid#30014DepositorInsertpLtetO-1:mCherry, pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT3A
Plasmid#79501PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDSDepositorInsertWNT3A (WNT3A Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHU6_gRNA_Ch10-for1
Plasmid#81210Purposefor targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch10
Plasmid#81224PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_gRNA_Ch10-rev1
Plasmid#81208Purposefor targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct:MCP-VP64
Plasmid#78904PurposeExpresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertMS2:VP64
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceMarch 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKalpha RP deletion
Plasmid#33328DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
UseTagsFLAGExpressionMammalianMutationDeleted RP domain (amino acids 167-189 missing)PromoterSV40Available sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
Dom34(1-138)
Plasmid#19101DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSR21
Plasmid#69156Purposeread-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr XDepositorInsertsmCherry
TagBFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 2818 (=HL 865 + pHL 489)
Plasmid#30032DepositorInsertpLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-deltaNLS1
Plasmid#22153DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationdeletion of amino acids 297-304PromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHL 1011
Plasmid#52993PurposepLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfpDepositorInsertsRhyB sRNA
RBS (st7)
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationPromoterpLlac0-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR
Plasmid#61779PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1DepositorInsertmouse Xbp1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pP2A11 (GB0082)
Plasmid#68168PurposeProvides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid partDepositorInsertSl2A11 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only