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Plasmid#166458PurposeExpresses fusion of mEGFP and YAPS127ADepositorInsertmEGFP, YAPS127A (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
A*11:01:01:01
Plasmid#203276PurposeOverexpress HLA alleleDepositorInsertA*11:01:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-E343A
Plasmid#171378PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationE343APromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::lacZ-H11
Plasmid#139099PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHsp68Available sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-Yap shRNA
Plasmid#166486PurposeMouse Yap RNAiDepositorInsertshRNA for mouse Yap (Yap1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-YAP1-shRNA1
Plasmid#176846Purposelentiviral vector for doxycycline-inducible human YAP1 knockdownDepositorInsertYAP1-Sh1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-Dsup-C
Plasmid#90022PurposeTo express GFP-tagged C-terminal region of Dsup in mammalian cells transientlyDepositorInsertDsup
UseTagsAcGFP1ExpressionMammalianMutationC-terminal region alonePromoterCMVAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 N C-V5-tag
Plasmid#179987PurposeMammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 N
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 N C-V5-tag
Plasmid#179968PurposeMammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 N (N Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-KKKAAA
Plasmid#171219PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.DepositorInsertAR-KKKAAA (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-K630A-K632A-K633APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 ORF3a C-V5-tag
Plasmid#179988PurposeMammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 ORF3a
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EmGFP-LATS2/1 KD
Plasmid#52085PurposeLentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporterDepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D
Plasmid#177320PurposeLentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).DepositorInsertKRAS 4B (KRAS Human)
UseLentiviralTagsFKBP-derived dimerization domain (DD, or DmrB) an…ExpressionMammalianMutationG12DPromoterTetOn-CMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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LV-AR-C563A-C596A
Plasmid#171220PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.DepositorInsertAR-C563A-C596A (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-C563A-C596APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro
Plasmid#176850Purposelentiviral vector for doxycycline-inducible YAP1 overexpressionDepositorInserthYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationfull length YAP1PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-YAP1-shRNA2
Plasmid#176847Purposelentiviral vector for doxycycline-inducible human YAP1 knockdownDepositorInsertYAP1-Sh2 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL A WT
Plasmid#202413PurposeExpression of GFP-tagged PODXL (isoform A) WTDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-R599A-N600A
Plasmid#171221PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.DepositorInsertAR-R599A-N600A (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-R599A-N600APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N-EP300
Plasmid#166452PurposeExpresses fusion of mCherry and EP300-N-IDRDepositorInsertmCherry, N-EP300(1-341) (EP300 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only