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pYTK-DN6

Plasmid

#180287

Purpose

HygR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - HygR - CEN6/ARS4

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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iRFP670-PIP4K2C

Plasmid

#202750

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP670:PIP4K2C (PIP4K2C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMCB306

Plasmid

#89360

Purpose

sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

GFP, puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB6576

Plasmid

#106134

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0

Plasmid

#153288

Purpose

Express the adenosine sensor GRAB_Ado1.0 in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Tags

Expression

Mutation

Promoter

GfaABC1D

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pME-BrainbowTEC

Plasmid

#82405

Purpose

Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Inserts

tdTomatoMYC fusion
EGFP
E2CrimsonHA

Use

Tags

HA and MYC

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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NW50

Plasmid

#133445

Purpose

Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW50

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pYTK-DN5

Plasmid

#180286

Purpose

NatR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - NatR - CEN6/ARS4

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-8xbiRhoBAST

Plasmid

#196351

Purpose

Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-8xbiRhoBAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

Plasmid

#196936

Purpose

TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

Depositor

Elaine Fuchs

Article

Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

Insert

mCherry

Use

Lentiviral and RNAi

Tags

P2A-CreERT2

Expression

Mammalian

Mutation

Promoter

SBE

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTAT-CRE

Plasmid

#35619

Depositor

Steven Dowdy

Article

Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

Insert

CRE (cre Bacteriophage P1)

Use

Tags

6xHIS, T7 tag, and TAT

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-hph-Ptac

Plasmid

#218364

Purpose

Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-neo Flag HAUSP

Plasmid

#16655

Depositor

Bert Vogelstein

Article

Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

Insert

HAUSP (USP7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pAV0761

Plasmid

#133494

Purpose

Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDae029 (eMutaT7[TadA-8e])

Plasmid

#187620

Purpose

Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

TadA-8e-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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