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Plasmid#231920PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134, N1158, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G12345 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1134Q, N1158Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G1345 mutant
Plasmid#231919PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G1345 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1158Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHPHS232
Plasmid#228356PurposeLanding pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locusDepositorInsertsmTagBFP2
Reverse tetracycline transactivator 3
neo
UseTagsBlasticidin S deaminase and iCasp9ExpressionMammalianMutationPromoterTRE3GV and gene trapAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-PCNT
Plasmid#227284PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.DepositorInsertsgRNA Targeting N-terminus of PCNT (PCNT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1616A/S1691A
Plasmid#215909PurposeExpression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1332D/S1346D
Plasmid#215912PurposeExpression of full-length TNRC6A with S1332D/S1346D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1332D, S1346DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1332A/S1346A
Plasmid#215913PurposeExpression of full-length TNRC6A with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1332A, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1616D/S1691D
Plasmid#215914PurposeExpression of full-length TNRC6A with S1616D/S1691D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616D, S1691DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-FL-S1616A/S1691A
Plasmid#215915PurposeExpression of full-length TNRC6A with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationFull length of TNRC6A, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A
Plasmid#215900PurposeExpression of TNRC6A-CIM1 domain with S1332A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1332APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A
Plasmid#215901PurposeExpression of TNRC6A-CIM1 domain with S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A/S1346A
Plasmid#215902PurposeExpression of TNRC6A-CIM1 domain with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1332A/S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A
Plasmid#215903PurposeExpression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709, S1332A, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-S1616A/S1691A
Plasmid#215904PurposeExpression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709, S1616A, S1691APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D
Plasmid#215906PurposeExpression of TNRC6A-silencing domain (SD) with S1332D/S1346D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1332D, S1346DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A
Plasmid#215907PurposeExpression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1332A, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1616D/S1691D
Plasmid#215908PurposeExpression of TNRC6A-silencing domain (SD) with S1616D/S1691D mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1616D, S1691DPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV272
Plasmid#226183PurposeExpresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF130 shRNA
Plasmid#225340PurposeshRNAmir backbone for RNA interference, with YFPDepositorInsertRNF130 shRNA (Rnf130 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-MYD88-ts3
Plasmid#174282PurposeMYD88 knockdownDepositorInsertMYD88 shRNA (MYD88 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only