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pEJS1089: mini-AAV.sgRNA.Nme2Cas9

Plasmid

#159536

Purpose

Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with single guide RNA cassette

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE3G-GSX2-IRES2-EBF1

Plasmid

#96966

Purpose

Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)

Use

Tags

FLAG (on EBF1)

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-WT-puro

Plasmid

#156166

Purpose

Lentiviral vector for expression of RAP1A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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lenti.DTR.GFP

Plasmid

#201962

Purpose

Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

Depositor

Bernhard Schmierer

Article

Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

Insert

Diptheria Toxin Receptor

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG SARS-CoV2 RdRp.co

Plasmid

#154008

Purpose

Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

Depositor

Patrick Moore

Article

Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

Insert

Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)

Use

Tags

3xFLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PLKO.1-YAP shRNA2

Plasmid

#166485

Purpose

Human YAP RNAi

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

shRNA for human YAP (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAP

Plasmid

#166457

Purpose

Expresses fusion of mEGFP and YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-C-EP300

Plasmid

#166454

Purpose

Expresses fusion of mCherry and EP300-C-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, C-EP300(1795-2414) (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP

Plasmid

#166443

Purpose

Bacterial expression of 6x His tagged YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Mito-Pericam

Plasmid

#87381

Purpose

Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

Depositor

Björn Stork

Article

Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

Insert

Mito-Pericam [ratiometric]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Retroviral 5' LTR promoter

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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A*02:02:01:01

Plasmid

#203277

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:02:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 C-V5-tag ORF3a

Plasmid

#179969

Purpose

Mammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF3a (ORF3a Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

Plasmid

#207880

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 M C-V5-tag

Plasmid

#179978

Purpose

Mammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 M (M Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.KD.flag

Plasmid

#19759

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

D173A (kinase dead)

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only
pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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