-
Plasmid#194289PurposeMultisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQVDepositorInsertHyper7
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE4
Plasmid#195369PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInserthA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-tubulin
Plasmid#136573PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670-tubulin
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE5
Plasmid#195371PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8DepositorInsertecTadA8e-(GGGGS)x8-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag7
Plasmid#169330PurposeExpression of TOMM20-HaloTag7 in mammalian cellsDepositorInsertTOMM20-HaloTag7 (TOMM20 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
Plasmid#20942Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsExpressionMammalianMutationH134R mutation in ChR2PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV_nLightG
Plasmid#217656PurposeExpresses norepinephrine indicator nLightG under mammalian promoterDepositorInsertnLightG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICS PM-ER Short P2A
Plasmid#164112PurposeDetect the short Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_TetO_CD19-P2A-mCherry
Plasmid#133803Purposerepressible expression of CD19 and mCherry in mammalian cellsDepositorInsertputative ECD of H. sapiens CD19 (CD19 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHFR-SpCas9-DHFR
Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.col…ExpressionMammalianMutationPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
2xP53_RE::FLuc
Plasmid#178316PurposeP53 Firefly luciferase reporterDepositorInsertP53 FLuc Reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPLICS PM-ER Long P2A
Plasmid#164111PurposeDetect the long Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xPP7_SL]
Plasmid#68424PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertINT construct bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-VP64 dCas9 VP64-T2A-GFP
Plasmid#59791PurposeCo-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFPDepositorInserthumanized VP64 dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM4-Halo-FRB
Plasmid#197057PurposeExpression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM7-Halo-FRB
Plasmid#197058PurposeExpression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM22-Halo-FRB
Plasmid#197059PurposeExpression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_FRB-GEM23-Halo
Plasmid#197060PurposeExpression of nanocage I3-01 Halo-FRB-fusion in mammalian cellsDepositorInsertI3-01
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only