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myc-Orai1 E190A pHAGE

Plasmid

#22753

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E190A (ORAI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E190A

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
pAJ12

Plasmid

#14914

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

GFP(S65C)

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAJ14

Plasmid

#14916

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAJ15

Plasmid

#14917

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pHis-TEV-Nsp15

Plasmid

#187656

Purpose

E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

Depositor

Debra Hansen

Article

Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

Insert

Nsp15 (N Severe acute respiratory syndrome coronavirus 2)

Use

Tags

TEV protease cleavable His6-tag

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-HKRA-sgRNA_v7

Plasmid

#208357

Purpose

Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-HKRA

Use

Tags

Expression

Mammalian

Mutation

I123H/D195K/D208R/V232A

Promoter

CMV

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo-YAP

Plasmid

#166468

Purpose

Plasmid coding for YAP and neomycin resistance in a lentiviral backbone.

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP, Neo (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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B*40:01:01

Plasmid

#203257

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:01:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3

Plasmid

#75127

Purpose

Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-YHAM-sgRNA_v7

Plasmid

#208356

Purpose

Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-YHAM

Use

Tags

Expression

Mammalian

Mutation

F48Y/S188H/V232A/E316M

Promoter

CMV

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

Plasmid

#172640

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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SF-SON IRES Puro

Plasmid

#214402

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Shh

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pF CAG luc-EGFP-cre puro

Plasmid

#67503

Purpose

Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

luciferase-EGFP-cre

Use

Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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SF-SON IRES Tomato

Plasmid

#214403

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1096 Dual-sgRNA.Design 1

Plasmid

#159538

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 nuclease with two guide RNA cassettes with promoters

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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