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Plasmid#213406PurposePeroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)DepositorInsertβ2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEG302-JMJ14-ZF
Plasmid#200913PurposeExpress JMJ14-ZF108 in Arabidopsis target FWA geneDepositorInsertJMJ14
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEG302-HD2A-ZF
Plasmid#200915PurposeExpress HD2A-ZF108 in Arabidopsis target FWA geneDepositorInsertHD2A
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_MCS_version1
Plasmid#197937PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.DepositorInsertpcoCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
UseTags6 his tagExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15 PemrR-gfp biosensor
Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
UseTagsEGFPExpressionBacterialMutationPromoterPemrRAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-K630A-K632A
Plasmid#171263PurposeMammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)DepositorInsert3xFlag-tagged human androgen receptor Lys630Ala-Lys632Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Lys630Ala-Lys632Ala (AR-K630A-K632A)PromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 deltaTPR mutant
Plasmid#67614Purposebacterial expression of GST-PRMT9 missing residues 21-139DepositorInsertPRMT9 (PRMT9 Human)
UseTagsGSTExpressionBacterialMutationtruncation mutant missing aa 21-139PromotertacAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 deltaN (aa 21-350)
Plasmid#67611Purposebacterial expression of GST-PRMT9 missing residues 21-350DepositorInsertPRMT9 (PRMT9 Human)
UseTagsGSTExpressionBacterialMutationtruncation mutant missing aa 21-350PromotertacAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
UseTagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-GFP
Plasmid#58977Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xHA-hTERT
Plasmid#51637PurposeMammalian expression of 3xHA tagged human TERT, N-terminal.DepositorInsertTERT (TERT Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd nog
Plasmid#115488PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 nog
Plasmid#120251PurposeCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-Pontin
Plasmid#51635PurposeMammalian expression of 3xFLAG tagged pontin, N-terminal.DepositorInsertPontin (RUVBL1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-GFP-puroR
Plasmid#167463Purpose3rd-gen lentiviral expression of FLAG-GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
DYSF-Venus
Plasmid#29768DepositorInsertDYSF-Venus (DYSF Human)
UseTagsVenusExpressionMammalianMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi
Plasmid#199808Purposevector for constitutive CRISPRi-mediated gene knockdown in S. aureusDepositorInsertsdCas9
sgRNA with GFP cassette
UseCRISPRTagsExpressionMutationPromoterP23 and PRAB17 (sgRNA) and SarAP1 (GFP)Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-TERT
Plasmid#51631PurposeMammalian expression of 3xFLAG tagged TERT, N-terminal.DepositorInsertTERT (TERT Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB-wt
Plasmid#58905Purposemammalian expression of wild type human RbDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only