Addgene

We narrowed to 11,160 results for: cel.2

Showing: 6201 - 6220 of 11160 results

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pTRE3G-ZSGreen1-mHP1alpha

Plasmid

#181913

Purpose

Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Use

Tags

ZSGreen1

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro

Plasmid

#25936

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

Plasmid

#186967

Purpose

Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

DNMT3L (DNMT3L Human, Synthetic)

Use

Lentiviral

Tags

rTetR fusion

Expression

Mammalian

Mutation

Promoter

EF-1alpha promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF66 200-429-2xStrep

Plasmid

#130955

Purpose

Expresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Tags

2xStrep

Expression

Mammalian

Mutation

ORF66 a.a. 200-429

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly(S455D)

Plasmid

#70768

Purpose

Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Use

Tags

myc-epitope

Expression

Mammalian

Mutation

S455D

Promoter

EF1alpha

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly(S455A)

Plasmid

#70767

Purpose

Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Use

Tags

myc-epitope

Expression

Mammalian

Mutation

S455A

Promoter

EF1alpha

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IL6-Fc(DAPA)-AviTag-6xHis

Plasmid

#156587

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IL6 (IL6 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187288

Purpose

Express pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH A740D, E758K, RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJH3626

Plasmid

#179630

Purpose

Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Pacr-2(s)

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-TM

Plasmid

#176886

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

Ig kappa-FRISZ-PDGFR TM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.V842I

Plasmid

#81722

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I655V; V842I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.R897Q

Plasmid

#81714

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I655V; R897Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AcGFP-MSH2

Plasmid

#215121

Purpose

Expresses AcGFP-MSH2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MSH2 (MSH2 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM45_WT

Plasmid

#82892

Purpose

Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_WT

Plasmid

#82894

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-hMALAT1

Plasmid

#118580

Purpose

to overexpress human MALAT1 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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ATG9A-EGFP

Plasmid

#210367

Purpose

Expresses ATG9A fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

ATG9A (ATG9A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 YFP

Plasmid

#84910

Purpose

expression of YFP in mammalian cells

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

YFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.0

Plasmid

#176543

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.0
Cas12f-GE ver4.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and chicken β-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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