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Fibronectin-human-mutated V2 O-Glycosylation site

Plasmid

#120405

Purpose

enables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serine

Depositor

Inaam Nakchbandi

Article

Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

Insert

Fibronectin (FN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Contains complete variable region with a mutation…

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF1-R310_RASA2-EF1-Puro

Plasmid

#114097

Purpose

Express mutant RASA2 in mamalian cells

Depositor

Yardena Samuels

Article

Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

Insert

RASA2 (RASA2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R310*

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pVZ_PcoaT_SQE1_THAS1

Plasmid

#103859

Purpose

Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803

Depositor

Ilka Axmann

Article

Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

Insert

thalianol synthase 1 (THAS1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

inserted genes are codon optimized. 2nd amino aci…

Promoter

PcoaT (from Synechocystis sp. PCC 6803)

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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sgEML4

Plasmid

#85568

Purpose

Expresses sgRNA targeting human EML4 between exon 13 and 14

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

echinoderm microtubule associated protein like 4 (EML4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF17

Bacterial Strain

#102801

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF18

Bacterial Strain

#102802

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
pEMS1199

Plasmid

#29100

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple53 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1110

Plasmid

#29039

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple48 (DBH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1590

Plasmid

#29269

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple119 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 21, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1505

Plasmid

#29300

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple34 (CLDN5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1375

Plasmid

#29176

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple88 (GFAP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1506

Plasmid

#29251

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple35 (CLDN5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1540

Plasmid

#29262

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple69 (FEZF2 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1200

Plasmid

#29101

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple54 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1377

Plasmid

#29178

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple90 (GFAP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1418

Plasmid

#29214

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple112 (HCRT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1524

Plasmid

#29255

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple53 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1595

Plasmid

#29272

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple124 (ICMT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1153

Plasmid

#29068

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple36 (CRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1473

Plasmid

#29234

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple3 (ADORA2A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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