Addgene

We narrowed to 11,160 results for: cel.2

Showing: 6181 - 6200 of 11160 results

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pPB-Neo-COVGT2-d2EGFP-mCherry

Plasmid

#201452

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT2_d2EGFP-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT4-d2EGFP-mCherry

Plasmid

#201453

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT4_d2EGFP-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT5-d2EGFP-mCherry

Plasmid

#201454

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT5_d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBA407-Neo-COVGT5-mCherry

Plasmid

#201455

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT5_mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc

Plasmid

#200127

Purpose

Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-D412AN414A_R

Plasmid

#147428

Purpose

Insect Expression of DmCCR4-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-D412AN414A (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-2xStrep-ORF66 1-200

Plasmid

#130954

Purpose

Expresses KSHV ORF66 a.a. 1-200 with an N-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Tags

2xStrep

Expression

Mammalian

Mutation

ORF66 a.a. 1-200

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1beta

Plasmid

#124140

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Marius Sudol, Megan Finch-Edmondson

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-hGRAD

Plasmid

#207837

Purpose

Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

mCherry
hFBXW11 (FBXW11 Human)
VHH-GFP4

Use

Tags

Expression

Mammalian

Mutation

215 amino acids of the full length protein

Promoter

TRE3G BI promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1beta

Plasmid

#181901

Purpose

Fluorescently tagged HP1beta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1beta (Cbx1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hNPM

Plasmid

#181887

Purpose

Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hNPM (NPM1 Human, Mustard Weed)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hNCL

Plasmid

#181886

Purpose

Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hNCL (NCL Human, Mustard Weed)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro

Plasmid

#25949

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A Δ450-572/Δ820-870-pBabe-Puro

Plasmid

#25955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α bHLH* P405A/P531A Δ820-870-pBabe-Puro

Plasmid

#25957

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

Plasmid

#64314

Purpose

chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

Depositor

Noriyuki Tsumaki

Article

Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

Inserts

COL11A2 promoter (COL11A2 Human)
COL11A2 enhancer (COL11A2 Human)

Use

Lentiviral

Tags

EGFP and IRES-PURO

Expression

Mammalian

Mutation

N/A

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCB268

Plasmid

#53363

Purpose

pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

Depositor

Alexander Varshavsky

Article

Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

Inserts

DHFR (Dhfr Mouse)
Ub
Aβ42 (APP Human)

Use

Tags

13xMyc, Flag, and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pUb FLAG-Mts D85N

Plasmid

#208403

Purpose

Expresses FLAG-tagged Drosophila mts protein with D85N mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

D85N

Promoter

Ubi-p63e

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H59Q

Plasmid

#208402

Purpose

Expresses FLAG-tagged Drosophila mts protein with H59Q mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

H59Q

Promoter

Ubi-p63e

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H241A

Plasmid

#208404

Purpose

Expresses FLAG-tagged Drosophila mts protein with H241A mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

H241A

Promoter

Ubi-p63e

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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