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Plasmid#200901PurposeExpresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.DepositorInsertPseTnsA-TnsB
UseTagsInternal NLS between TnsA-TnsB fusionExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI-NLS. PGK-puro
Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.tgoGAP1
Plasmid#78737PurposeExpression of Ca2+ sensor GAP1 in the trans Golgi apparatusDepositorInserttgoGAP1
UseTagsN terminal 81 amino acids of human galactosyltran…ExpressionMammalianMutationD117A/D119A/D163A in aequorin moietyPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
OMMGFP 1-10
Plasmid#164119PurposeExpresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMMDepositorInsertOMMGFP 1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisExpressionMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Luciferase
Plasmid#99283PurposeAll-in-one lentivector with tet-inducible luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAAV.GFAP.SV40.eGFP.SV40(polyA)
Plasmid#195558PurposeExpresses eGFP under control of short GFAP promoterDepositorInserteGFP
UseAAVTagsNo tagsExpressionMammalianMutationPromotershort GFAPAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSecTag-ATX
Plasmid#17839DepositorInsertAutotaxin (ENPP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK0_003
Plasmid#123925PurposeEncodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK systemDepositorInsertCas9-sgRNA destination
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LBR-FKBP-GFP
Plasmid#222413PurposeTo express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR (LBR Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKmyc-Borg3
Plasmid#141074PurposeExpresses Borg3 (Cdc42ep5) in mammalian cellsDepositorInsertBorg3 (Cdc42ep5 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM73
Plasmid#133651PurposeIntegration of CHS5-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-CHS5-mCherry (CHS5 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-C5E-6XTUBE
Plasmid#217628PurposeBacterial expression of MBP tagged 6XTUBEDepositorInsert6XTUBE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet30b-5M SrtA
Plasmid#51140Purposeexpression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 5M
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1
Plasmid#121488PurposeExpression of mouse PD-L1 WTDepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_TetO_CD19-P2A-mCherry
Plasmid#133803Purposerepressible expression of CD19 and mCherry in mammalian cellsDepositorInsertputative ECD of H. sapiens CD19 (CD19 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only