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Plasmid#87775PurposeExpresses V5 tagged human NIPP1DepositorInsertNIPP1 (PPP1R8 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos2-LaminB1-10
Plasmid#57395PurposeLocalization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64
Plasmid#101110PurposeEncodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2DepositorInsertBDKRB2-dCas9(C)_DHFR-PCP-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shNUP37 v1 puro
Plasmid#192343PurposeLentiviral expression vector for an inducible shNUP37 v1DepositorInsertshNUP37 v1 (NUP37 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shNUP37 v2 puro
Plasmid#192344PurposeLentiviral expression vector for an inducible shNUP37 v2DepositorInsertshNUP37 v2 (NUP37 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_SulR(CDS)
Plasmid#186423Purposeprovides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)DepositorInsertSulR
UseSynthetic Biology; Cds (part b3-b4-b5), goldenbra…TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PDCD6IP_BRO1
Plasmid#109994PurposeProtein expression and purification of PDCD6IP_BRO1DepositorInsertPDCD6IP_BRO1 (PDCD6IP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-LaminB1-10
Plasmid#54140PurposeLocalization: Nuclear Envelope, Excitation: 487, Emission: 509DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCINeoFlag ALIX
Plasmid#89859PurposeExpresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tmem214 pmRFP-N2 (1348)
Plasmid#62047Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTmem214 (TMEM214 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorInsertALFY (WDFY3 Human)
UseTags6xHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-UBE4B
Plasmid#113499PurposeExpression of human UBE4B with N-terminal GFP tagDepositorInsertUBE4B (UBE4B Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro BRIT1
Plasmid#16205DepositorInsertBRIT1 (MCPH1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_3XNLS-MicroID2-FLAG
Plasmid#188523PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cellsDepositorInsert3XNLS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-FLAG-NES
Plasmid#188525PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tmem214 pEGFP-N2 (1072)
Plasmid#62026Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTmem214 (TMEM214 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cdk6-DN-HA
Plasmid#1869DepositorInsertCdk6 (CDK6 Human)
UseTagsHAExpressionMammalianMutationDominant negative, D to N in conserved KLAD*FGLAR…PromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_2XMTS-MicroID2-FLAG
Plasmid#188524PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cellsDepositorInsert2XMTS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLG-bio-His
Plasmid#53427PurposeExpresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPLG (PLG Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
UseTagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only