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Plasmid#186379PurposeDestination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDEST-Spe4-Kozak-mCherry-RfA
Plasmid#186378PurposeDestination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Snaptag-RfA
Plasmid#186385PurposeDestination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-HAV_3C
Plasmid#203481PurposeExpresses HAV 3C protease from a GALL promoter with a URA3 markerDepositorInsertHAV 3C protease
UseTagsExpressionYeastMutationPromoterGALLAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-LV_3CL
Plasmid#203507PurposeExpresses LV 3CL protease from a GAL promoter with a URA3 markerDepositorInsertLV 3CL protease
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-SHV_3CL
Plasmid#203473PurposeExpresses SHV 3CL protease from a GAL10 promoter with a URA3 markerDepositorInsertSHV 3CL protease
UseTagsExpressionYeastMutationPromoterGAL10Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HHV-3_PR
Plasmid#203505PurposeExpresses protease domain of HHV-3 (VZV) from a GAL promoter with a URA3 markerDepositorInsertHHV-3 protease domain
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-NWV_3CL
Plasmid#203470PurposeExpresses NWV 3CL protease from a GAL10 promoter with a URA3 markerDepositorInsertNWV 3CL protease
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HTLV_PR
Plasmid#203467PurposeExpresses HTLV protease from a GAL10 promoter with a URA3 markerDepositorInsertHTLV protease
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Halotag-RfA
Plasmid#186377PurposeDestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Snaptag-stop
Plasmid#186395PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Halotag-stop
Plasmid#186387PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pANY8
Plasmid#164900PurposeContaining His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.DepositorInsertccdB cassette
UseTagsHis-ELP-Intein-tagExpressionBacterialMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EMCV_3C
Plasmid#201941PurposeExpresses EMCV 3C protease from a GAL promoter with a URA3 markerDepositorInsertEMCV 3C protease (EMCVgp1 )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mScarlet-I-stop
Plasmid#186393PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-PV_3C
Plasmid#203472PurposeExpresses PV 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertPV 3C protease (PVgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-1x-HIV-2_PR
Plasmid#201935PurposeExpresses HIV-2 protease from a GAL-1x promoter with a URA3 markerDepositorInsertHIV-2 protease
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mScarlet-I-RfA
Plasmid#186383PurposeDestination vector for the expression of a fluorescent fusion of N-ter mScarlet-I and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTD56
Plasmid#172895PurposemKate2^SEC^AID* vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTD72
Plasmid#172896PurposemKate2^SEC^2xHA vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only