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  1. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  9. Donor-PIGA-NeoR

    Plasmid
    #153549
    Purpose
    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Expression
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153549-plasmid-map-sequence-id-319273

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Showing: 601 - 620 of 736 results