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Plasmid#197053PurposepUBC-NLS-HA-2xMCP-1xGFP-eRF3DepositorInsertpubc-nls-ha-MCP-GFP-eRF3
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cdk9-Halo donor
Plasmid#113113PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequenceDepositorInsertCdk9 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPL5071_TEF1*-Cre_URA3
Plasmid#60930PurposeConstitutive expression vector for Cre-recombinase containing URA3 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-IPT-9
Plasmid#197746PurposePhytobrick (MoClo) Level 0 PartDepositorInsertIsopentyl transferase (IPT)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTF101.2
Plasmid#134771PurposeA binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLA230
Plasmid#11724DepositorInsertb-lactamase
UseTagsExpressionBacterialMutationGAA for Glu26 changed to TAA (premature stop codo…PromoterAvailable sinceAug. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
bactin2:loxP-STOP-loxP-DsRed-express
Plasmid#68797PurposeFor making transgenic fishDepositorInsertsbeta-actin promoter
loxp-stop-codon-loxp-dsred
UseTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#82400PurposeGateway-compatible zebrafish elavl3 promoterDepositorInsertelavl3 promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGST2-TSG101 (2-145)
Plasmid#184810PurposeBacterial expression of TSG101 UEV domain (aa 2-145)DepositorInsertTSG101 (TSG101 Human)
UseTagsGSTExpressionBacterialMutationaa 2-145 onlyPromotertacAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-8xbiRhoBAST-24xMS2v6
Plasmid#196352PurposeExpression of mAzurite mRNA with 8xbiRhoBAST-24xMS2v6 in 3'UTRDepositorInsertmAzurite-8xbiRhoBAST-24xMS2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-FnCas12a
Plasmid#113432PurposeExpresses 10xHis-MBP fused to FnCas12aDepositorInsertFnCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
BASIC_11_RBS34-GFP-B0015
Plasmid#68145Purposestorage vector for BASIC part: RBS-GFP(ORF)DepositorInsertRBS-34-GFP-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_201
Plasmid#91105PurposeTransformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT58_X2site
Plasmid#177705PurposeExpresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmGFP
Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
UseTags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmp8a
Plasmid#163592PurposeExpresses BMP-8A in mammalian cellsDepositorInsertBmp8a (Bmp8a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS220
Plasmid#177642PurposeIn vitro transcription of Patiria miniata DishevelledDepositorInsertDishevelled
UseIn vitro transcriptionTagsGFPExpressionMutationK47APromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBMCS-SortA
Plasmid#21931DepositorInsertsortase (srtA Staphylococcus aureus (strain N315))
UseTagsGB1 and His-tagExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_USP10_C424A
Plasmid#127103DepositorInsertUSP10 (USP10 Human)
UseTagsExpressionMammalianMutationC424APromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only