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pcDNA3.3_SARS2_BQ.1.1
Plasmid#194493Purposeexpressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus productionDepositorInsertSpike (BQ.1.1) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationR346T, K444T, N460K and c-terminal 18aa del on th…PromoterCMVAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACt-mCherry-NES
Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorInsertsACt-mCherry-NES (Adcy10 Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCW empty vector
Plasmid#184708PurposeLentiviral empty vector for doxycycline-inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mVenus C1
Plasmid#27794DepositorHas ServiceCloning Grade DNAInsertmVenus C1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLS-mP-Luc
Plasmid#106253PurposeLuciferase lentivirus based enhancer assay vectorDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterDerived from pGL4.23Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
ITIH4-bio-His
Plasmid#52181PurposeExpresses full-length Inter-alpha-trypsin inhibitor heavy chain H4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITIH4 (ITIH4 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+HyPer7-NES
Plasmid#136467PurposeMammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsNuclear export signal from the HIV Rev proteinExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUltra-MDH1-ME1
Plasmid#184465PurposeLentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)DepositorUseLentiviralTagsfused to T2AExpressionMammalianMutationdeletion of stop codonPromoterUbCAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BRAC/pcDNA3
Plasmid#51967Purposemammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicatorDepositorInsertBRAC
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCD564
Plasmid#153026PurposedxCas9(3.7), MCP-SoxS(R93A/S101A)DepositorInsertdxCas9(3.7), MCP-SoxS(R93A/S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFPC1-huNFATc1EE-WT
Plasmid#24219DepositorInsertNFATc1 (NFATC1 Human)
UseTagsEE and GFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCLX-UBI-mCherry
Plasmid#114275PurposeUbiquitous expression of the mCherry living colorDepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL2
Plasmid#101911PurposeExpresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7+LacOAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only
