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Showing: 6161 - 6180 of 147462 results

  6. pCMT-flp

    Plasmid
    #67274
    Purpose
    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis
    Depositor
    Mark Liles
    Article
    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-
    Insert
    tetA, flp, repA101, oriR101
    Use
    Flp recombinase plasmid, temperature sensitive
    Tags
    His-SUMO (flp)
    Expression
    Mutation
    Promoter
    tetA promoter of plasmid pACYC184 origin
    Availability
    Academic Institutions and Nonprofits only
    67274-plasmid-map-sequence-id-115886

    Enlarge

  7. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid
    #67275
    Purpose
    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do
    Insert
    puromycin resistant gene
    Use
    Tags
    linked to red flouresent protein gene (katushka)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    67275-plasmid-map-sequence-id-118496

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  9. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Use
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    67277-plasmid-map-sequence-id-121189

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  10. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    mTQ2=mturquoise2 blue fluorescent gene
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mutation
    Promoter
    Villin
    Availability
    Academic Institutions and Nonprofits only
    67278-plasmid-map-sequence-id-115887

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  11. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Mutation
    Promoter
    pdx
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

    Enlarge

  13. pMalAnPL

    Plasmid
    #67281
    Purpose
    Expresses MBP-Anacystis nidulans photolyase in E. coli
    Depositor
    Aziz Sancar
    Insert
    photolyase Anacystis nidulans
    Use
    Tags
    maltose binding protein
    Expression
    Bacterial
    Mutation
    none known
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    67281-plasmid-map-sequence-id-324478

    Enlarge

  15. p64PLA5

    Plasmid
    #67284
    Purpose
    6/4 photolyase gene from Drosophila in pFastbac vector, intermediate step in insect cell expression
    Depositor
    Aziz Sancar
    Article
    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.
    Insert
    6/4 photolyase from Drosophila
    Use
    Intermediate for insect expression
    Tags
    Flag
    Expression
    Mutation
    none known
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  16. pFBCPDL6

    Plasmid
    #67285
    Purpose
    CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression
    Depositor
    Aziz Sancar
    Article
    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.
    Insert
    CPD photolyase (phr Fly)
    Use
    Bacterial vector, insert not expressed
    Tags
    Flag
    Expression
    Mutation
    V275E*
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  17. pET28b(+)-TM0848

    Plasmid
    #67287
    Purpose
    Expresses TM0848 in E. coli
    Depositor
    SeWon Suh
    Insert
    GTP-binding protein YqeH
    Use
    Tags
    N tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 6161 - 6180 of 147462 results