Addgene

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pnEA-NpG-SpDcp1opt-Y36A_AD

Plasmid

#148542

Purpose

Bacterial Expression of SpDcp1opt-Y36A

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt-Y36A (dcp1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpScp1opt-K47A_AD

Plasmid

#148543

Purpose

Bacterial Expression of SpDcp1opt-K47A

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt-K47A (dcp1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-SpDcp2opt_1-242_AD

Plasmid

#148545

Purpose

Bacterial Expression of SpDcp2opt_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242 (dcp2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-SpDcp2opt_1-242-E147Q_AD

Plasmid

#148546

Purpose

Bacterial Expression of SpDcp2opt_1-242-E147Q

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242-E147Q (dcp2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-SpDcp2_1-266_X

Plasmid

#148007

Purpose

Bacterial Expression of SpDCP2_1-266

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP2_1-266 (dcp2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-SpDCP2_1-242_X

Plasmid

#148008

Purpose

Bacterial Expression of SpDCP2_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP2_1-242 (dcp2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-SpDCP2_1-253_X

Plasmid

#148009

Purpose

Bacterial Expression of SpDCP2_1-253

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDCP2_1-253 (dcp2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-SpEDC3_1-94_X

Plasmid

#148005

Purpose

Bacterial Expression of SpEDC3_1-84

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpEDC3_1-84 (edc3 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-mVenus

Plasmid

#194000

Purpose

Transient expression of AtOEP7-mVenus in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mVenus

Use

Tags

AtOEP7 1–50aa

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtTIM21(1-50aa)-mCherry

Plasmid

#194047

Purpose

Transient expression of AtTIM21-mCherry in plant cell (Mitochondria)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mCherry

Use

Tags

AtTIM21 1–50aa

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-Zic4

Plasmid

#193009

Purpose

Plasmid to generate an ISH probe for Hydra Zic4

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Zic4

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Zic4-3505:GFP

Plasmid

#193001

Purpose

Plasmid to generate a transgenic Hydra Zic4 reporter line

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Zic4 promoter

Use

Hydra transgenesis vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Zic4-3505:Luc

Plasmid

#193002

Purpose

Plasmid to test the Hydra Zic4 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Zic4 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-NematocilinA

Plasmid

#193010

Purpose

Plasmid to generate an ISH probe for Hydra Nematocilin A

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Nematocilin A

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-LexAOx4-Pmin-mCherry

Plasmid

#192633

Purpose

Lentiviral vector for inducible mCherry expression.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Minimal promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-HT-emiRFP703

Plasmid

#183985

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

H2B (H2BC11 Human)

Use

Tags

HaloTag, emiRFP703

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-HT-mGold

Plasmid

#183988

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

H2B (H2BC11 Human)

Use

Tags

HaloTag, mGold

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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Sth_I-E array_GFP dropout

Plasmid

#178775

Purpose

Golden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

gfp dropout to generate S. thermophilus type I-E single arrays

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm2_A

Plasmid

#145887

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm5_A

Plasmid

#145890

Purpose

Insect Expression of DmLsm5

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLsm5 (CG6610 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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