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Showing: 6141 - 6160 of 154173 results
  1. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pYPQ265E2

    Plasmid
    #164719
    Purpose
    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing
    Depositor
    Insert
    A3A(Y130F)-zCas9(D10A)-UGI
    Use
    CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. TFORF2240

    Plasmid
    #141984
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    GATA3 (GATA3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pET_BGL1

    Plasmid
    #101910
    Purpose
    Expresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.
    Depositor
    Insert
    β-glucosidase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7+LacO
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV Retrograde)

    Viral Prep
    #162377-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Promoter
    Syn
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pCRISPR-cBEST

    Plasmid
    #125689
    Purpose
    C to T base editor for actinomycetes
    Depositor
    Insert
    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    ermE*/tipA
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. SETD1A

    Plasmid
    #32868
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    SETD1A (SETD1A Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid
    #169247
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.
    Depositor
    Insert
    RiboL1-jGCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pRKO

    Plasmid
    #49456
    Purpose
    loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions
    Depositor
    Insert
    loxP-kanMX-loxP cassette
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
  11. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF

    Plasmid
    #163317
    Purpose
    Encodes for first COVID protein - NSP1, NSP10, NSP11, and fragment of NSP12 fused to nano-luciferase
    Depositor
    Insert
    Nsp1, Nsp10, Nsp11 ,Nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Luciferase
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 6141 - 6160 of 154173 results