221,577 results

1. CAV1-mCherry

   Plasmid

   #27705

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Tags

   monomeric Cherry

   Expression

   Mammalian

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAV1-mCherry

   Plasmid

   #27705

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Tags

   monomeric Cherry

   Expression

   Mammalian

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mycBioID2-13X Linker-MCS

   Plasmid

   #92308

   Purpose

   To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Humanized A. aeolicus BioID2 with R40G and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD57

   Plasmid

   #119882

   Purpose

   Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pConstitutive_GFP+
   

   Expression

   Bacterial

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET32a-hEGF

   Plasmid

   #199231

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Epidermal growth factor, partial (EGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA 3 5' cMyc SMC1 wt

   Plasmid

   #32363

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Tags

   c-Myc

   Expression

   Mammalian

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA 3 5' cMyc SMC1 wt

   Plasmid

   #32363

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Tags

   c-Myc

   Expression

   Mammalian

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRecT_2

   Plasmid

   #167546

   Purpose

   Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Insert

   E. faecium Com15 recT under an IPTG inducible promoter, with lacI
   

   Expression

   Bacterial

   Promoter

   IPTG

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-fDIO mCherry (AAV8)

   Viral Prep

   #114471-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   mCherry (Flp-dependent)

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

10. Mito-TurboID

    Plasmid

    #200960

    Purpose

    Transiently expresses mitochondria localized TurboID with V5 tags

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    mito-TurboID
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

    Plasmid

    #164611

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Expression

    Mammalian

    Mutation

    S383T

    Promoter

    CMV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mito-BFP

    Plasmid

    #49151

    Purpose

    visualization of mitochondria

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    mitochondrial targeting signal (COX4 Budding Yeast)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mito-BFP

    Plasmid

    #49151

    Purpose

    visualization of mitochondria

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    mitochondrial targeting signal (COX4 Budding Yeast)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LifeAct-mGarnet2

    Plasmid

    #104308

    Purpose

    codes for a fusion of fluorescent protein mGarnet2 and lifeact that targets F-actin in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

    Insert

    LifeAct-mGarnet2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.GfaABC1D.PI.Lck-GFP.SV40

    Plasmid

    #105598

    Purpose

    AAV expression of membrane bound EGFP from GfaABC1D promoter

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Has Service

    AAV5

    Insert

    Lck-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-GFP (AAV PHP.eB)

    Viral Prep

    #37825-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pAdx-CMV-tdTomato

    Plasmid

    #73347

    Purpose

    Express tdTomato under CMV promoter

    Depositor

    Kazuhiro Oka

    Insert

    CMV-tdTomato
    

    Use

    Adenoviral

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAdx-CMV-tdTomato

    Plasmid

    #73347

    Purpose

    Express tdTomato under CMV promoter

    Depositor

    Kazuhiro Oka

    Insert

    CMV-tdTomato
    

    Use

    Adenoviral

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dual-sgRNA_hU6-mU6

    Plasmid

    #154194

    Purpose

    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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