169,069 results

1. PH-Akt-GFP

   Plasmid

   #51465

   Purpose

   Is a biosensor for PIP3/PI(3,4)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   AKT (aa 1–164)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV CMV-dSaCas9-KRAB-bGHpA

   Plasmid

   #106219

   Purpose

   AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA-3xFLAG-Pontin

   Plasmid

   #51635

   Purpose

   Mammalian expression of 3xFLAG tagged pontin, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   Pontin (RUVBL1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-NES-FRCaMPi

   Plasmid

   #232838

   Purpose

   AAV transfer plasmid for Syn-mediated expression of FRCaMPi

   Depositor

   Kiryl Piatkevich

   Article

   Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

   Insert

   NES-SomaFRCaMPi (NES Synthetic)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-ERBB2-V5/HIS

   Plasmid

   #201103

   Purpose

   expression of human ERBB2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. β3-integrin-YFP

   Plasmid

   #26653

   Depositor

   Jonathan Jones

   Article

   Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

   Insert

   β3-integrin (ITGB3 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHTNW

   Plasmid

   #136403

   Purpose

   Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

   Plasmid

   #158741

   Purpose

   Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Hemagglutinin A/MI/45/15 H1N1
   

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-ZO1-C-14

    Plasmid

    #55165

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    ZO1 (TJP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJYS1Ptac

    Plasmid

    #85545

    Purpose

    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Promoter

    tac and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti NbALFA-mNeonGreen puro

    Plasmid

    #193973

    Purpose

    lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-mNeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBoBi-hTRPV4-C-GC6s

    Plasmid

    #154150

    Purpose

    To detect the calcium ion releases from TRPV4 channel

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

    Insert

    TRPV4 and GCamp6s (TRPV4 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCB320

    Plasmid

    #89359

    Purpose

    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    puromycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-hERalpha

    Plasmid

    #101141

    Purpose

    Mammalian expression of full-length human estrogen receptor-alpha

    Depositor

    Elizabeth Wilson

    Article

    Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

    Insert

    estrogen receptor-alpha (ESR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPSup

    Plasmid

    #149416

    Purpose

    Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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