168,974 results

1. pCAGGS_cAMPFIRE-L

   Plasmid

   #182283

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-L (cAMP sensor)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TGFB1_pLX307

   Plasmid

   #98377

   Purpose

   Lentiviral expression of TGFB1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TGFB1 (TGFB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBR1k-con-GFPuv

   Plasmid

   #106386

   Purpose

   Constitutive trc promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet3-Giantin_C1

   Plasmid

   #189773

   Purpose

   Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-Giantin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-HA-USP39

   Plasmid

   #22581

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP39 (USP39 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

6. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

   Plasmid

   #221235

   Purpose

   Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
   

   Use

   Lentiviral

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mOrange2-pBAD

   Plasmid

   #54531

   Purpose

   Localization: Protein Expression Vector , Excitation: 549, Emission: 565

   Depositor

   Michael Davidson, Nathan Shaner, Roger Tsien

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Tags

   6xHis and mOrange2

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

   Plasmid

   #128605

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   24xSunTag
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-Tre3G-29xGGGGCC-12xMS2

    Plasmid

    #99149

    Purpose

    Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

    Depositor

    Ron Vale

    Article

    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

    Insert

    29xGGGGCC 12xMS2 hairpins
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-eGFP

    Plasmid

    #162823

    Purpose

    Doxycycline-inducible overexpression of eGFP

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V163A

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hIBA1a-GFP-miR124T (AAV5)

    Viral Prep

    #214147-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hIBA1a-GFP-miR124T (#214147). In addition to the viral particles, you will also receive purified pAAV-hIBA1a-GFP-miR124T plasmid DNA. hIBA1a-driven and miR124T-targeted for microglial inhibition of GFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Tags

    GFP

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

13. pHIV-EGFP

    Plasmid

    #21373

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

    Depositor

    Bryan Welm, Zena Werb

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM5411

    Plasmid

    #132663

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Expression

    Bacterial

    Promoter

    PaadA and PconII

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-Soma-jGCaMP8f (AAV1)

    Viral Prep

    #169258-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn-Soma-jGCaMP8f (#169258). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8f plasmid DNA. Synapsin-driven neuronal expression of soma-targeted calcium sensor jGCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

16. V5-APEX2-LMNA

    Plasmid

    #129275

    Purpose

    Expression of APEX2 at nuclear lamina

    Depositor

    Alice Ting

    Article

    Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

    Insert

    V5-APEX2-LMNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_BiSSTe10_dTomato_nlsdTomato (AAV PHP.eB)

    Viral Prep

    #213814-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV_BiSSTe10_dTomato_nlsdTomato (#213814). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the SST interneuron-targeting enhancer E10. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Tags

    dTomato

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

18. pMPRA1

    Plasmid

    #49349

    Purpose

    Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRIME-TET-GFP-FF3

    Plasmid

    #11662

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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