168,579 results

1. pMSR:MSMEG_1353:Dendra

   Plasmid

   #175002

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_1353
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Promoter

   pmycTetO

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSR:MSMEG_0250:Dendra

   Plasmid

   #159047

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0250
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Promoter

   pmycTetO

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRuby2-C1

   Plasmid

   #54768

   Purpose

   Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

   Plasmid

   #87829

   Purpose

   High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   MTS
   NLS
   PTS1
   

   Expression

   Mammalian

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

   Plasmid

   #175525

   Purpose

   Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10 and Tomm20-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-(BbsI)_CBh-Cas9-T2A-BFP

   Plasmid

   #64323

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-EBFP2

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

   Plasmid

   #40343

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   RSV promoter
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   RSV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-syn-jGCaMP7s-WPRE (AAV PHP.eB)

   Viral Prep

   #104487-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pMH-SFB

   Plasmid

   #99391

   Purpose

   Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Synthetic, Human)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mNeonGreen-mRuby2-FRET-10

    Plasmid

    #58179

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

    Type

    Empty backbone

    Tags

    mNeonGreen and mRuby2

    Expression

    Mammalian

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-c2X

    Plasmid

    #75286

    Purpose

    Expresses proteins in the cytoplasm as fusions to maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 18, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

    Plasmid

    #164514

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.

    Depositor

    Loren Looger

    Article

    Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Mutation

    Synthetic Construct

    Promoter

    GFAP

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C2-NLRP3

    Plasmid

    #73955

    Purpose

    Express EGFP-tagged human NLRP3 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV iE

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV Retrograde)

    Viral Prep

    #162379-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid

    #100843

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ141B2.0

    Plasmid

    #99897

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-GFP-IRES-CRE

    Plasmid

    #48201

    Purpose

    retroviral vector encoding for GFP and Cre-recombinase

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Inserts

    eGFP
    Cre
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG and linked by IRES

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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