168,515 results

1. U6-3' MS2-CircRNA

   Plasmid

   #213751

   Purpose

   For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

   Plasmid

   #162230

   Purpose

   Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   LaG17 GFP nanobody SynNotch tTA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-SLC35G6_STOP

   Plasmid

   #161262

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35G6 (SLC35G6 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. ACS1

   Plasmid

   #13746

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   Acetyl CoA Synthetase 1 (Acss2 Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1_V5_TurboID_FLAG_Nt

   Plasmid

   #194072

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5-TurboID-FLAG

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CAG-jGCaMP8m-WPRE (AAV9)

   Viral Prep

   #179255-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CAG

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

7. pLX307/PTPN11

   Plasmid

   #140941

   Purpose

   expresses PTPN11

   Depositor

   Kimberly Stegmaier

   Article

   Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

   Insert

   PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_sfCherry2(1-10)

   Plasmid

   #82602

   Purpose

   Expresses sfCherry2(1-10) fusion in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(1-10)
   

   Expression

   Mammalian

   Mutation

   changed Glu 118 to Gln, changed Thr 128 to Ile

   Promoter

   CMV

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-DIO-eYFP (AAV1)

   Viral Prep

   #117383-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-TRE-DIO-eYFP (#117383). In addition to the viral particles, you will also receive purified pAAV-TRE-DIO-eYFP plasmid DNA. Tet-inducible, Cre-dependent eYFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Promoter

   TRE

   Tags

   eYFP (Tet-inducible, Cre-dependent)

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

10. pENTR223-BALF4

    Plasmid

    #195835

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF4 (BALF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-GFP

    Plasmid

    #160697

    Purpose

    Expresses GFP in mammalian cells

    Depositor

    Christine Mayr

    Article

    Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p18S.2 tag

    Plasmid

    #51731

    Purpose

    transcribes truncated transcript containing 18S and hybridization tag

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    18S
    

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAL4-DBD-MCS

    Plasmid

    #145245

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF5B-FRT-GFP-αTAT1[D157N]

    Plasmid

    #27100

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Tags

    GFP and S-tag

    Expression

    Mammalian

    Mutation

    D157N

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe_Hyg_cOVA_T2A_mStrawberry

    Plasmid

    #161737

    Purpose

    Expression of cOVA and mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    cytoplasmic ovalbumin
    

    Use

    Retroviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-Hygro-EF1A-EGFP-PARP1

    Plasmid

    #176146

    Purpose

    EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Anti-VGAT [L118/80R]

    Plasmid

    #177463

    Purpose

    Mammalian Expression Plasmid of anti-VGAT (Mouse). Derived from hybridoma L118/80.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VGAT (Mus musculus) recombinant mouse monoclonal antibody (Slc32a1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-cMSPdH45

    Plasmid

    #199767

    Purpose

    Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH45
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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