169,099 results

1. pLJM1-FLAG-GFP-Tmem192

   Plasmid

   #134630

   Purpose

   lysosomal marker

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP15745: pAAV-AiE0452h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5745)

   Plasmid

   #230880

   Purpose

   AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   mScarlet
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIP-GFP1-10

   Plasmid

   #68715

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   GFP1-10
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4-Chk1-Flag

   Plasmid

   #22894

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Chk1 (CHEK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_hSyn1-SIO-stGtACR1-FusionRed

   Plasmid

   #105678

   Purpose

   Expresses a soma-targeted GtACR1 in Cre-expressing neurons

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV5

   Insert

   stGtACR1
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE30-His-CKM

   Plasmid

   #124134

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Creatine kinase (CKM Chicken)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

   Plasmid

   #208692

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3m
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1 CCNE1

    Plasmid

    #164144

    Purpose

    Doxycycline inducible expression of Cyclin E1

    Depositor

    Richard Possemato

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. psfaMs-Tn5

    Plasmid

    #183637

    Purpose

    Expresses anti-mouse nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti mouse secondary nanobody fused to Tn5
    

    Tags

    anti-mouse nanobody

    Expression

    Bacterial

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV9)

    Viral Prep

    #112005-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Promoter

    Syn

    Tags

    mRuby3

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

14. PH-Akt-GFP

    Plasmid

    #51465

    Purpose

    Is a biosensor for PIP3/PI(3,4)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CamKII-ArchT-GFP (PV2527) (AAV5)

    Viral Prep

    #99039-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition.

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Promoter

    CaMKII

    Tags

    GFP

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEN2-SG4

    Plasmid

    #196071

    Purpose

    Express wild type SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4
    

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. His6-MBP-uTEV3

    Plasmid

    #135464

    Purpose

    Bacterial expression of uTEV3 (full-length) protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    His6-MBP-uTEV3
    

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability) , I138T/S153N…

    Promoter

    T5

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-sCAG-mBaoJin-oPRE

    Plasmid

    #218684

    Purpose

    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Short variant of the CAG promoter

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a-HisRS-His

    Plasmid

    #124111

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Histidyl-tRNA ligase (hisS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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