168,579 results

1. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

   Plasmid

   #242657

   Purpose

   CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA, eVRQR mutations in SpCas…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-28a(+)-FGF2-K128N

   Plasmid

   #120284

   Purpose

   Produce human recombinant FGF2 K128N in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-Rab4B

   Plasmid

   #61801

   Purpose

   Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab4B (RAB4B Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pF141 pAcGFP1 SOD1WT

   Plasmid

   #26402

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216246

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgAAVS1.5
   

   Use

   CRISPR; Human safe harbor locus (a avs1) site-spe…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMAH-POLY-eRF1(E55D)

   Plasmid

   #157925

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

   Insert

   POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMET17p-CRU313

   Plasmid

   #131163

   Purpose

   For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Tags

   CRU

   Expression

   Yeast

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQFT-mNG

   Plasmid

   #203675

   Purpose

   pQFT with mNeonGreen under control of PQJ

   Depositor

   Urs Jenal

   Article

   Klotz et al Appl Environ Microbiol. 2023 May 18:e002

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Promoter

   PQJ

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS-U6-crRNA-empty

   Plasmid

   #134921

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   crRNA for CRISPR-Cas3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPR132-Tango

    Plasmid

    #66315

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR132 (GPR132 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-TRE-fDIO-GFP-IRES-tTA

    Plasmid

    #118026

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-fDIO-GFP-IRES-tTA
    

    Use

    AAV

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro

    Plasmid

    #176850

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 overexpression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    full length YAP1

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Klf2-t2A-mCherry

    Plasmid

    #127539

    Purpose

    Expresses mouse Klf2 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Klf2 (Klf2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-HA-SAMTOR

    Plasmid

    #100513

    Purpose

    Expresses HA tagged SAMTOR in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-SEpHluorin

    Plasmid

    #32001

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    mCherry
    

    Tags

    Superecliptic pHluorin

    Expression

    Mammalian

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO-cell-mCer

    Plasmid

    #185601

    Purpose

    Lentiviral construct for expression of mCerulean

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    cell-mCer
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tg(mpeg1:LifeAct-mRuby)

    Plasmid

    #166977

    Purpose

    labels F-actin in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

    Inserts

    mpeg1 (mpeg1.1 Zebrafish)
    LifeAct (actb1 Frog)
    

    Tags

    Lifeact-mRuby and mRuby

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50463

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti PGK Blast DEST (w524-1)

    Plasmid

    #19065

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge