168,499 results

1. p8.91

   Plasmid

   #187441

   Purpose

   Lentiviral packaging plasmid expressing gag and pol.

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Inserts

   Use

   Lentiviral

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-mApple

   Plasmid

   #179383

   Purpose

   Lentiviral vector for expression of mApple

   Depositor

   Angus Johnston

   Article

   Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

   Insert

   mApple
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGK-T7/2-CD44s (fl)

   Plasmid

   #137812

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44s (fl) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length, I186T and I253V on CD44

   Promoter

   PGK

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR::rpsL

   Plasmid

   #44505

   Purpose

   A crRNA expression plasmid specific to the rpsL allele.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR::rpsL (rpsL )
   

   Use

   CRISPR; E.coli

   Available Since

   April 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. HcKCR2_mCherry_pcDNA3.1

   Plasmid

   #177337

   Purpose

   Expresses KCR2 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR2
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mammalian codon-adapted

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Halo-MTS

   Plasmid

   #124315

   Purpose

   Expresses mitochondria specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Tags

   Mito specific seq

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. All_in_one_CRISPR/Cas9_LacZ

   Plasmid

   #74293

   Purpose

   "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

   Depositor

   Lynne Postovit

   Article

   Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

   Inserts

   LacZ-alpha
   Cas9
   mCherry
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   Lac promoter, SV40, and T7 promoter

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYC-C

   Plasmid

   #207114

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCS-13X Linker-BioID2-HA

   Plasmid

   #80899

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_CMV: Flag-Akaluc_SV40:PuroR

    Plasmid

    #183048

    Purpose

    Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    AkaLuc
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 18065-M01-412

    Plasmid

    #225664

    Purpose

    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsCopGFP
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    SORE6-mCMVp and WPRE-SV40p

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL056

    Plasmid

    #177168

    Purpose

    Expresses wildtype MRE11 in human cells

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCG-Hc∆18

    Plasmid

    #84817

    Purpose

    Measles virus hemagglutinin expressing plasmid

    Depositor

    Jakob Reiser

    Article

    Marino et al Gene Ther. 2015 Mar;22(3):280-5. doi: 10

    Insert

    Measles virus H glycoprotein
    

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcmv3-IRG1-HA

    Plasmid

    #198181

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1)

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    HA tag is added to C-terminal of target protein

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231346

    Purpose

    Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHIV-H2B-eGFP

    Plasmid

    #91776

    Purpose

    Expresses H2B protein tagged with eGFP

    Depositor

    Maria Pia Cosma

    Article

    Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

    Insert

    Human Histone H2B / eGFP (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep

    #100833-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pGP-AAV-syn-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep

    #104487-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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