168,838 results

1. pORANGE Cacnb3-GFP KI

   Plasmid

   #139662

   Purpose

   Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12222_U6-pegRNA-BFP

   Plasmid

   #177181

   Purpose

   pegRNA cloning plasmid with BFP marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Mutation

   VP64 activator fusion; NLS fusion

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. BirA-Myc_N_PAX6

   Plasmid

   #167721

   Purpose

   Expresses PAX6 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   PAX6 (PAX6 Human)
   

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE50

   Plasmid

   #90389

   Purpose

   PPARg/RARa - PPARg gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   ACO1

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-LiMETER-v2

   Plasmid

   #113934

   Purpose

   Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

   Depositor

   Yubin Zhou

   Article

   He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

   Insert

   LOV2 and Stim1 polybasic domain (PB)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-myc-FLNa WT

   Plasmid

   #8982

   Depositor

   John Blenis

   Article

   Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

   Insert

   Filamin A (FLNA Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. GHRHR-Tango

   Plasmid

   #66292

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GHRHR (GHRHR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBAD-pHlorina

   Plasmid

   #182763

   Purpose

   pH-sensitive RFP pHlorina for detection of efferocytosis

   Depositor

   Robert Campbell

   Article

   Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

   Insert

   pHlorina
   

   Expression

   Bacterial

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200630

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Promoter

    pTRE3G

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frt-hspB5

    Plasmid

    #63096

    Purpose

    Expression of HSPB5 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. AXBEv2

    Plasmid

    #204606

    Purpose

    Mammalian expression of evolved adenine transversion base editor AXBEv2

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid

    #127368

    Purpose

    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid

    #123235

    Purpose

    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 Human, aequorea victoria)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-SMARCA4

    Plasmid

    #65391

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCM184

    Plasmid

    #46012

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Type

    Empty backbone

    Use

    Cre/Lox

    Promoter

    na

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. p300 KAT LIC 1M

    Plasmid

    #233587

    Purpose

    Bacterial expression of p300 KAT enzyme

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300 KAT (EP300 Human)
    

    Tags

    6xHis MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES-hrGFP II-TET1

    Plasmid

    #83568

    Purpose

    Expresses TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65252

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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