168,461 results

1. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. REM1

   Plasmid

   #55681

   Purpose

   expression of mouse REM1

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   REM1 (Rem1 Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pHR_PGK_ anti-PTPRZ CA domain 13058 (VL+VH) synNotch

   Plasmid

   #247517

   Purpose

   Expresses a synNotch receptor binding to PTPRZ

   Depositor

   Wendell Lim

   Article

   Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

   Insert

   Expresses a synNotch receptor binding to PTPRZ
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR_PGK_ anti-PTPRZ CA domain 13059 (VL+VH) synNotch

   Plasmid

   #247519

   Purpose

   Expresses a synNotch receptor binding to PTPRZ

   Depositor

   Wendell Lim

   Article

   Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

   Insert

   Expresses a synNotch receptor binding to PTPRZ
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR_PGK_ anti-NRCAM 12949 (VL + VH) synNotch

   Plasmid

   #247525

   Purpose

   Expresses a synNotch receptor binding to NRCAM

   Depositor

   Wendell Lim

   Article

   Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

   Insert

   Expresses a synNotch receptor binding to NRCAM
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CAG-CitA-Tq-HR

   Plasmid

   #241833

   Purpose

   Mammalian expression vector for CitA-Tq-HR

   Depositor

   Gary Yellen

   Article

   Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

   Insert

   CitA-Tq-HR
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-CitA-Tq-LR

   Plasmid

   #241832

   Purpose

   Mammalian expression vector for CitA-Tq-LR (low-range)

   Depositor

   Gary Yellen

   Article

   Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

   Insert

   CitA-Tq-LR
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CMV-RBP2

   Plasmid

   #241990

   Purpose

   Mouse RBP2 (accession ID NP_001074857.1), CMV promoter-enhancer, downstream WPRE.

   Depositor

   Tobias Moser

   Article

   Kapoor et al eLife13:RP98254

   Insert

   RIM-BP2 (Rimbp2 Mouse)
   

   Use

   AAV

   Tags

   RIM-BP2 (untagged)

   Expression

   Mammalian

   Promoter

   CMV enhancer

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-HBA-RIBEYE-eGFP

   Plasmid

   #241988

   Purpose

   Human RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance

   Depositor

   Tobias Moser

   Article

   Kapoor et al eLife13:RP98254

   Insert

   RIBEYE-GFP (CTBP2 Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   hybrid CMV-enhancer-human-beta-actin promoter (CM…

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJP_Osc05

    Plasmid

    #229040

    Purpose

    Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.

    Depositor

    Yolanda Schaerli

    Article

    Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

    Insert

    pBAD promoter
    

    Expression

    Bacterial

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFUW-TetO-ZEB2

    Plasmid

    #207203

    Purpose

    doxycycline-inducible expression of Human ZEB in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-ZEB2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV_hSyn1_SypHer3s_cyto

    Plasmid

    #238971

    Purpose

    Mammalian neurons SypHer3s expression

    Depositor

    Dmitry Bilan

    Article

    Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

    Insert

    SypHer3s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_GFAP_SypHer3s_cyto

    Plasmid

    #238969

    Purpose

    Mammalian astrocytes SypHer3s expression

    Depositor

    Dmitry Bilan

    Article

    Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

    Insert

    SypHer3s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

    Plasmid

    #187076

    Purpose

    Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-dHaloTag7_T2A_Lifeact-HaloTag7
    

    Expression

    Mammalian

    Mutation

    dHaloTag7 = HaloTag7-D106A

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFR(L858R,T790M)-NanoLuc Fusion Vector

    Plasmid

    #237199

    Purpose

    Express EGFR(L858R,T790M)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    EGFR (EGFR Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    L858R/T790M double mutations

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAAV-hSyn-tdTomato

    Plasmid

    #239048

    Purpose

    AAV construct used as a cell filler to label neurons with tdTomato

    Depositor

    Erin O'Shea

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCOLA-A-pC-tac

    Plasmid

    #213494

    Purpose

    Encodes Node A of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    rpaI (rpaI )
    tetR
    4cl
    tal
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-mNeptune2-SKL

    Plasmid

    #183729

    Purpose

    For exrpression of mNeptune2 targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    mNeptube2
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Promoter

    UbiC

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSII42N-TDH3pr

    Plasmid

    #194531

    Purpose

    High copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSETA-qmTQ2-cAMP

    Plasmid

    #225249

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge