168,493 results

1. pCRISPaint-mNeon-PuroR

   Plasmid

   #174090

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   mNeonGreen
   

   Use

   Gene tagging

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp3 -EGFP

   Plasmid

   #165108

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp3 (ORF1ab SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-MTOR-S2215Y

   Plasmid

   #69013

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2215Y

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP-TRF1 pWzl-Hygro

   Plasmid

   #19834

   Depositor

   Titia de Lange

   Article

   Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

   Insert

   Telomere-Repeat binding Factor 1 (Terf1 Mouse)
   

   Use

   Retroviral

   Tags

   enhanced GFP

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

5. MSCV

   Plasmid

   #24828

   Depositor

   Lin He

   Article

   Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3Basic-ME.2/ApoEpromoter

   Plasmid

   #51436

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   APOE apolipoprotein E (APOE, ME2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The ME.2 enhancer is fused upstream of the ApoE g…

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB_smFLAG_KDM5B_MS2

   Plasmid

   #81084

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_KDM5B_24xMS2
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-FLAG-CaMKIIA

   Plasmid

   #186825

   Purpose

   Expresses FLAG-CaMKIIA in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2a (Camk2a Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-sgR26

   Plasmid

   #127376

   Purpose

   Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC25A39

    Plasmid

    #131970

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A39 (SLC25A39 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCITF-KCC2

    Plasmid

    #61404

    Purpose

    Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

    Depositor

    Franck Polleux

    Article

    Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

    Insert

    KCC2 (SLC12A5 Human)
    

    Tags

    IRES-tdTomato-Flag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid

    #188383

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAT2

    Plasmid

    #112583

    Purpose

    Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBE48

    Plasmid

    #174656

    Purpose

    CAPTURE method BAC origin of replication receiver plasmid

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SypHer2-DAAO-NES

    Plasmid

    #119184

    Purpose

    Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

    Depositor

    Thomas Michel

    Article

    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

    Insert

    SypHer2-DAAO-NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-VSVG

    Plasmid

    #64084

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV glycoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV5)

    Viral Prep

    #162381-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-mGold2s-Calnexin-N-14

    Plasmid

    #231768

    Purpose

    Visualization of ER in mammalian cells using mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s-Calnexin-N-17 (CANX Human)
    

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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