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Plasmid#124252PurposeIn vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV129
Plasmid#124253PurposeIn vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJWK_VD_04
Plasmid#158105PurposeCaV1.3 5'UTR luciferase reporterDepositorInsert5'UTR of CACNA1D (uc003dgu.5)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_03
Plasmid#158104PurposeCaV1.2 5'UTR luciferase reporterDepositorInsert5'UTR of CACNA1C (uc009zdu.1)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorInsertshCrh(2) (Crh Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-AuroraB L154A/H250Y
Plasmid#108491Purposeexpression of EGFP-AuroraB Analog sensitive (L154A/H250Y)DepositorInsertAuroraB (AURKB Human)
UseTagsEGFPExpressionMammalianMutationAnalog sensitive L154A/H250YPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482-GFP
Plasmid#108500Purposeinducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFPDepositorInsertINCENP d543-746iPRC1 304-509 (PRC1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationdelta alpha Helix, insert PRC1 microtubule domainPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP
Plasmid#108503Purposeinducible expression of vsv-INCENP d543-746-EGFPDepositorInsertINCENP d543-746 (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationdelta alpha helixPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp d543-746iPRC1_279-482-GFP
Plasmid#108504Purposeinducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFPDepositorInsertINCENP d543-746iPRC1 304-509 (PRC1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationdelta alpha Helix, insert PRC1 microtubule domainPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP
Plasmid#108505Purposeinducible expression of vsv-INCENP 8KR-EGFPDepositorInsertINCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationK548R, K563R, K602R, K609R, K614R, K622R, K630R, …PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP
Plasmid#108506Purposeinducible expression of vsv-INCENP 8KQ-EGFPDepositorInsertINCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationK548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Incenp 1-48-GFP
Plasmid#108507Purposeexpression of vsv-INCENP 1-48-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Incenp 1-58-GFP
Plasmid#108508Purposeexpression of vsv-INCENP 1-58-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Incenp 1-63-GFP
Plasmid#108509Purposeexpression of vsv-INCENP 1-63-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only