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Plasmid#201975PurposeExpress a pegRNA used for G to T conversion at HEK4 siteDepositorInsertpegRNA_HEK4_G-T
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_HEK4_1bp-deletion
Plasmid#201976PurposeExpress a pegRNA used for 1bp deletion at HEK4 siteDepositorInsertpegRNA_HEK4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B4
Plasmid#172845PurposeCRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mNeonGreen
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track HA PGC-1 alpha
Plasmid#14427DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsHAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-Lrat
Plasmid#206345PurposeAAV plasmid expressing LRAT in photoreceptorsDepositorInsertLrat (Lrat Mouse)
UseAAVTagsExpressionMutationPromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bmi1 shRNA1
Plasmid#21576DepositorInsertBmi-1 (Bmi1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B3
Plasmid#172844PurposeCRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mTurquoise2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mTagBFP2-P2A-EGFP
Plasmid#184943PurposeExpresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cellsDepositorInsertmTagBFP2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTnsABC
Plasmid#160031PurposeExpresses V. cholerae TnsABCDepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonTagsExpressionBacterialMutationnoPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2-1
Plasmid#31828DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-CCSS
Plasmid#83424PurposeMouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidues 218 and 219 from C to S (CCSS: TGCTGT -&…PromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK4alpha WT
Plasmid#12187DepositorInsertMEKK4 (Map3k4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Gamma-Tubulin-17
Plasmid#58099PurposeLocalization: Centrosomes, Excitation: 554, Emission: 581DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A2
Plasmid#131891PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A2 (SLC6A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM6-RgTALsyn
Plasmid#100947PurposeExpression of Tyrosine Ammonia Lyase from inducible T7 promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPW3755 : HDR-HsIRE1a-10xGS-HaloTag
Plasmid#185677PurposeHDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.DepositorInsertERN1 homology arms (ERN1 Synthetic, Human)
UseCRISPRTagsHaloTagExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-TGAT
Plasmid#84334PurposeExpresses TGAT tagged with mCherryDepositorInsertTGAT (TRIO Human)
UseTagsmCherryExpressionMammalianMutationWTPromoterCMVAvailable sinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-D261A
Plasmid#31729DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutationChanged D261 to AlaninePromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HseIF4AII_AC
Plasmid#148439PurposeMammalian Expression of HseIF4AIIDepositorInsertHseIF4AII (EIF4A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only