We narrowed to 18,928 results for: CAG

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pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Publication

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Expression

Mammalian

Mutation

none

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

Plasmid

#108843

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

Plasmid

#108845

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GAI

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gfi1 (Gfi1 Mouse)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAGGS

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

Plasmid

#87584

Purpose

PV5 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP1 POD-nAb-WPRE

Plasmid

#244973

Purpose

Expresses a fusion protein of a nanobody against GFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Publication

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

GFP1 POD-nAb

Available since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pT/CAGGS-NRASG12V/D38A-IRES-mVenus

Plasmid

#236077

Purpose

Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).

Depositor

Masashi Narita

Publication

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Inserts

mVenus
NRAS G12V D38A (NRAS Human)

Use

Sleeping beauty transposon plasmid

Mutation

G12V D38A

Promoter

CAGGS

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PDGFRb559-562del-IRES2-EGFP

Plasmid

#204355

Purpose

Expresses a mutant PDGFRb gene (559-562 deletion).

Depositor

Hirofumi Nakatomi

Publication

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Mutation

559-562 deletion

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ULK1 (836-1050)-MBP

Plasmid

#203562

Purpose

Mammalian expression of ULK1 (836-1050) with MBP tag

Depositor

James Hurley

Publication

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ULK1 (ULK1 Human)

Tags

MBP-10His

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194723

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Publication

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

Plasmid

#161923

Purpose

To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

Depositor

Sébastien Britton

Publication

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting HSD17B11 exon 1

Use

CRISPR

Promoter

U6

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

Plasmid

#216321

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Publication

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice acceptor site

Use

AAV

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Publication

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTRIPZ (M)-HT-Cbx2

Plasmid

#82510

Purpose

Expresses Halotag-Cbx2 fusion proteins in mammalian cells

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 2 (CBX2 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Publication

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#87560

Purpose

Expresses aa397-423 of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa397-423

Use

Cre/Lox and Synthetic Biology

Tags

PYL

Expression

Mammalian

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#87559

Purpose

Expresses aa1-396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa1-396

Use

Cre/Lox and Synthetic Biology

Tags

ABI

Expression

Mammalian

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCXLE-dCas9VPH-T2A-GFP-shP53

Plasmid

#102895

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9VPH-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIPZ (M)-HT-Cbx2

Plasmid

#82510

Purpose

Expresses Halotag-Cbx2 fusion proteins in mammalian cells

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 2 (CBX2 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GSK3β-#1

Plasmid

#32496

Depositor

Alex Toker

Publication

Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

Insert

GSK3B (GSK3B Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles