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Plasmid#160073PurposeZ. mobilis CRISPRi, promoter A, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_StrepII_TEV_PP2Ac
Plasmid#217477PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tagDepositorInsertPP2Ac
UseTagsStrepII, TEVExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mhYFP
Plasmid#186532PurposeMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertMonomeric hyperfolder YFP
UseTags6xHisExpressionBacterialMutationhfYFP-S147P/L195M/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmGFP
Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
UseTags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro FLAG SREBP1
Plasmid#32017DepositorInsertSREBP1 (Srebf1 Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationV225MPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mhYFP
Plasmid#186533PurposeMonomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertMonomeric hyperfolder YFP
UseTagsExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG-W-Flag-Nbs1
Plasmid#89315Purposeretroviral expression of mouse Nbs1DepositorInsertNbs1 (Nbn Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-BsaI_pJMP2776
Plasmid#208890PurposeAcinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_US71_APikL1
Plasmid#123646PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertUS71_APikL1
UseGolden gateTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mhYFP
Plasmid#186524PurposeLocalization of monomeric hyperfolder YFP fluorescent protein to actinDepositorInsertMonomeric hyperfolder YFP
UseTagsLifeActExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-BsaI_pJMP2748
Plasmid#208888PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UsePir dependent origin of replicationTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-gfp_pJMP2768
Plasmid#208889PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LSSmGFP
Plasmid#186543PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cellsDepositorInsertLSSmGFP
UseTagsExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Tat HA (P#29)
Plasmid#14654DepositorInsertTat (tat HIV)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
H2B-LSSmGFP
Plasmid#186539PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2BDepositorInsertLSSmGFP
UseTagsHuman Histone 2B (D26G and V119I mutations)ExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mito-mhYFP
Plasmid#186526PurposeLocalization of monomeric hyperfolder YFP fluorescent protein to mitochondriaDepositorInsertMonomeric hyperfolder YFP
UseTagsHuman COX8a first 29 amino acidsExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3 CDK8 HA (P#634)
Plasmid#14649DepositorInsertCdk8 (CDK8 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSSmGFP
Plasmid#186534PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actinDepositorInsertLSSmGFP
UseTagsLifeActExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER2-mhYFP
Plasmid#186528PurposeLocalization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiencyDepositorInsertMonomeric hyperfolder YFP
UseTagsCalreticulin and KDELExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only