-
Plasmid#134404PurposeLight-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB105
Plasmid#68559PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB130
Plasmid#68584PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB108
Plasmid#68562PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-AAK1-V5_IDG-K
Plasmid#135257PurposeGateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAAK1-V5 (AAK1 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-USP21 (USP, aa 196-565)
Plasmid#61585PurposeExpresses human USP21 (USP domain) in E. coli.DepositorInsertUSP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
UseTagsHis6-SUMOExpressionBacterialMutationDeleted aa 1-195.PromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hMLKL
Plasmid#208789PurposeInducibly expresses human MLKL, fused to light-activatable Cry2DepositorInsertMLKL (MLKL Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTagsHIS-6-tag and Twin-Strep-tagExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNup133-Ypet
Plasmid#98283Purposemammalian expression of Nup133-YpetDepositorInsertNup133 (NUP133 Human)
UseTagsYPetExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCTAIRE-1-HA
Plasmid#1999DepositorInsertPCTAIRE-1 (CDK16 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB110
Plasmid#68564PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-S6K1(wt) barcoded
Plasmid#48801Purposeexpresses wt S6K1DepositorInsertS6K1 (Rps6kb1 Rat)
UseLentiviralTagsFLAG and barcode: GGATCCExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-Nup43
Plasmid#98276Purposemammalian expression of SNAPf-Nup43DepositorInsertNup43 (NUP43 Human)
UseTagsSNAPf-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_1
Plasmid#73525PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hSAPS1-HA
Plasmid#165466PurposeHuman SAPS1 mammalian expression vector with C-terminal HA tag.DepositorInsertProtein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Puro-iDEST
Plasmid#75336PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-CDK13_IDG-K
Plasmid#135268PurposeGateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-FLAG-mPKM2
Plasmid#44239DepositorInsertPyruvate Kinase M2 (Pkm Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2013AvailabilityAcademic Institutions and Nonprofits only