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Plasmid#68152Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -423 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.41 hA33 luciferase
Plasmid#68154Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -405 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.36 hA33 luciferase
Plasmid#68155Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -363 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.38 hA33 luciferase
Plasmid#68156Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -384 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.22 hA33 luciferase
Plasmid#68157Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -222 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-H2B-C-10
Plasmid#57789PurposeLocalization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertH2B (H2BC11 Human)
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR wt-FLAG
Plasmid#204525PurposeRetroviral expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1
Plasmid#184395PurposeDonor plasmid for stable integration of firefly luciferase control reporter at AAVS1DepositorInsertFirefly luciferase beta-globin
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-LDLR-mCherry BlastR
Plasmid#186739PurposeDox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertLDLR (LDLR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
Plasmid#127392PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acid and Threon…PromoterpCAGAvailable sinceJuly 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-Mito-7
Plasmid#57773PurposeLocalization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertMito (COX8A Human)
UseTagsminiSOGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV2-SEP-GluA1 (M1)
Plasmid#64942Purposemammalian expression of rat GluA1DepositorInsertGluA1 (Gria1 Rat)
UseTagsSEP (superecliptic pHluorin)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Cx43-GFP
Plasmid#176860PurposeExpresses Cx43 fused with GFP at the C-terminus in astrocytesDepositorInsertCx43-eGFP (Gja1 Mouse)
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GalNAc-T2-GFP-ESCargo(FTV)
Plasmid#140163PurposeExpresses a regulatable secretory cargo for mammalian cells and a Golgi markerDepositorInsertGalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 399 SNP flag pLenti4/To/v5/DEST
Plasmid#42651Purposedestination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cellsDepositorInsertTLR4 399 SNP (TLR4 Human)
UseLentiviralTagsFlagExpressionMammalianMutationT399IPromoterCMV/TOAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST
Plasmid#42650Purposedestination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cellsDepositorInsertTLR4 299 SNP (TLR4 Human)
UseLentiviralTagsFlagExpressionMammalianMutationD299GPromoterCMV/TOAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
LLPS1-iLID::EGFP::FTH1 (pBS1042)
Plasmid#185289PurposeFor the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertiLID::eGFP::FTH1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
INS-2A-luciferase-2A-CD19-GFP
Plasmid#159349PurposeHuman INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.DepositorInsertsUseIns locus knockin donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-hCD2-CD14 (4R)
Plasmid#133062PurposeRetroviral expression of mouse CD14 mutant "4R" deficient in LPS bindingDepositorInsertCD14 (4R) (Cd14 Mouse)
UseRetroviralTagsExpressionMammalianMutationCD14 allele defective for LPS binding (mutations …PromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-PDL1
Plasmid#159280PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInsertHuman PDL1/CD274 (CD274 Human)
UseAAV; S1 knockin donor vectorTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only