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Plasmid#44246PurposeExpression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdead Cas9 with 3X NLS
UseCRISPR and Retroviral; VectorTags3x NLSExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab5a-c-Myc
Plasmid#79801PurposeExpress TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).DepositorInsertRab5a (RAB5A Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CaMKII-JEDI-1P-Kv-WPRE
Plasmid#202614PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertJEDI-1P-Kv
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-GFP
Plasmid#58976Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab11a
Plasmid#79805PurposeExpress TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).DepositorInsertRab11a (RAB11A Human)
UseTagsTagBFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV_tKiMBI-T2A-caMEK
Plasmid#199578PurposeExpress tKiMBI and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-mAirn-R-loop
Plasmid#134900PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInsertmAirn promoter (Airn Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hSNRPN-R-loop
Plasmid#134902PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInserthSNRPN promoter (SNRPN Human)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
Plasmid#199585PurposeExpress tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA2
Plasmid#174667PurposeExpresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertGFP-endoNA2 fusion protein
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO mTRPM7
Plasmid#45482DepositorInsertTRPM7 (Trpm7 Mouse)
UseTetracycline inducibleTagsFlagExpressionMammalianMutationPromoterTetracycline-controlled CMVAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-Usp9x_CD-mCherry
Plasmid#170380PurposeVector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.DepositorInsertUbiquitin specific protease 9x catalytic domain (Usp9x Mouse)
UseTagsmCherry-3xFlagExpressionMammalianMutationCatalytic domainPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE
Plasmid#202617PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependentDepositorInsertFRT-JEDI-1P-Kv
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 K720A
Plasmid#19939DepositorInsertcullin 1 K720A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a NEDD8 modification mutantPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI.neo-rPAM1-M314I
Plasmid#145802Purposeexpresses catalytically inactive rat PAM-1 in mammalian cellsDepositorInsertrat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
UseTagsExpressionMammalianMutationchanged methionine 314 to isoleucine to inactivat…PromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-iGECI
Plasmid#160423PurposeAAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECIDepositorInsertiGECI
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H81
Plasmid#170339PurposeGFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertGFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-1P-WPRE
Plasmid#202611PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertJEDI-1P
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTag-RFP-C-h-Rab11a
Plasmid#79806PurposeExpress TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).DepositorInsertRab11a (RAB11A Human)
UseTagsTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
CanlonicSF
Plasmid#178342PurposeTo explore lactate dynamics in intact systemsDepositorInsertCanlonicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only