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pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

Plasmid

#179459

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV1

Insert

JEDI-2P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-2P-Kv-WPRE

Plasmid

#179463

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P-Kv

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 HxD

Plasmid

#136365

Purpose

Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

HxD (H1077Y, D1079A)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pT2-shATRX-GFP

Plasmid

#124259

Purpose

Expresses shRNA targeting ATRX with a GFP reporter which is driven by the SV40 promoter. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Expression

Mammalian

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

Plasmid

#179465

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-FHA-SHLD2

Plasmid

#114121

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1 hMOG-alpha2-EGFP

Plasmid

#160976

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha3-EGFP

Plasmid

#160977

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD2-m1

Plasmid

#114120

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pT2-shATRX

Plasmid

#124258

Purpose

Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Expression

Mammalian

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNTK Gatad2a flox

Plasmid

#110812

Purpose

Donor targeting vector for generating mouse Gatad2a conditional knockout allele

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Unspecified

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta3-EGFP

Plasmid

#160980

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta3 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

Plasmid

#209083

Purpose

SNCA (Avi tagged) expression along with BirA ligase

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
BirA

Tags

Avi

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGLUE-SHLD2

Plasmid

#114118

Purpose

Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Tags

HA tag, Streptavidin-binding tag (Streptag), and …

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-pLenti-ACTA2-R179H (LLH647)

Plasmid

#242683

Purpose

Plasmid for expression of ACTA2-R179H cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-R179H_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-R179H

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180M (LLH673)

Plasmid

#242686

Purpose

Plasmid for expression of ACTA2-L180M cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180M_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180M

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180V (LLH669)

Plasmid

#242685

Purpose

Plasmid for expression of ACTA2-L180V cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180V_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180V

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-D181G (LLH681)

Plasmid

#242687

Purpose

Plasmid for expression of ACTA2-D181G cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-D181G_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-D181G

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180L (LLH677)

Plasmid

#242688

Purpose

Plasmid for expression of ACTA2-L180L cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180L_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180L

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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