Addgene

147,462 results

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pMAL-c5X-poly-EAK9

Plasmid

#67010

Purpose

Expresses malE-poly-EAK9 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly-EAK9

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-CLP3.7

Plasmid

#67011

Purpose

Expresses malE-CLP3.7 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-CLP3.7

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[AV-60]

Plasmid

#67012

Purpose

Expresses malE-ELP[AV-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[AV-60]

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[V-60]

Plasmid

#67013

Purpose

Expresses malE-ELP[V-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V-60]

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pET-24a-ELP[V-30]

Plasmid

#67014

Purpose

Expresses ELP[V-30] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

ELP[V-30]

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET-24a-ELP[V-150]

Plasmid

#67015

Purpose

Expresses ELP[V-150] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

ELP[V-150]

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein beta-1

Plasmid

#67016

Purpose

G protein beta-1 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein beta-1 (GNB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein beta-2

Plasmid

#67017

Purpose

G protein beta-2 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

NM_005273.3 (GNB2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein gamma-2

Plasmid

#67018

Purpose

G protein gamma-2 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-2 (GNG2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pMXsIP GFP-VPS33A

Plasmid

#67022

Purpose

human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMRXIP VPS16-GFP

Plasmid

#67023

Purpose

Human VPS16 ORF was inserted into pMRXIP GFP-N vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pRJPaph-eBFP2

Plasmid

#67030

Purpose

Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Insert

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mTq2

Plasmid

#67031

Purpose

Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mTurqoise2

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-bjGFP

Plasmid

#67032

Purpose

Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-sYFP2

Plasmid

#67033

Purpose

Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
sYFP2

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mChe

Plasmid

#67034

Purpose

Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mCherry

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-HcRed

Plasmid

#67035

Purpose

Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
HcRed

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-gusA

Plasmid

#67036

Purpose

Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
gusA

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRJPaph-lacZYA

Plasmid

#67037

Purpose

Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
lacZYA

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
HaloTag-G protein gamma-1

Plasmid

#67038

Purpose

G protein gamma-1 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-1 (GNGT1 Bovine)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge