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Plasmid#98806PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV T17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
UseTagsExpressionBacterialMutationT17S, N68D, E107D, D127A, F132L, S135F, F162S, K2…PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pST50Trc2-HPCHISNDHFR
Plasmid#63966PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Protein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBACgus N-His/GST/LRRK2 1867-2176
Plasmid#40406DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867-2176PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc1-STRHISNDHFR
Plasmid#63946PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-FLAGHISNDHFR
Plasmid#64001PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-CBPNDHFR
Plasmid#63941PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCalmodulin Binding Peptide (CBP); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-FLAGNDHFR
Plasmid#63997PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
GRM3 (3SM9)
Plasmid#32870PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRM3 (GRM3 Human)
UseTagsHis tag with TEV cleavage and Honeybee melittin S…ExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
ABL2
Plasmid#39178PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertABL2 (ABL2 Human)
UseBaculovirus expressionTagsHis TevExpressionMutationTruncated (see comments)PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc2-FLAGHISNDHFR
Plasmid#63965PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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GRM7
Plasmid#28110PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRM7:DCC014-D04:C211307 (GRM7 Human)
UseTagsHis tag with TEV siteExpressionBacterialMutationcontains ligand binding domain, residues 37-513.PromoterAvailable sinceMarch 18, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus N-His/GST/LRRK2 1867-2186
Plasmid#40403DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867-2186PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIC.B4.SPy0289
Plasmid#11534DepositorInsertIscU
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET27b+R
Plasmid#188245PurposeEmpty backbone vector for protein expression in Escherichia coli and his-tag purificationDepositorTypeEmpty backboneUseTags6xHis tag and TEV siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-T4 Lysozyme C54T C97A (WT*)
Plasmid#224353PurposeExpresses T4 lysozyme in E. coliDepositorInsertT4 Lysozyme
UseTagsTEV-cleavable His6ExpressionBacterialMutationC45T, C94APromoterT5Available sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pGL4_10xUAS-CMVmin-BC0427-luc2
Plasmid#214634Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0427
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only