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Plasmid#15287DepositorInsertFGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
UseTagsMyristoylation-targeting domain c-Src (14aa) and …ExpressionMammalianMutationFGFR3 cytoplasmic domain only FKBP12 F36VPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVA (E1658A) pCAGGS
Plasmid#206082Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIA (E314A) pCAGGS
Plasmid#206081Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pcDNA3
Plasmid#206066Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pCAGGS
Plasmid#206096Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658KPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3
Plasmid#73208PurposeExpresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)DepositorInsertCOXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO mMecp2-NLucTom
Plasmid#159282PurposeTo tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.DepositorInsertMecp2 (Mecp2 Mouse)
UseTagsNluciferase-P2A-TdTomatoExpressionMammalianMutationNluciferase-TdTomato fusionPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-196a-2SM-PGK-GFP-Blast
Plasmid#31512DepositorInsertmiR-196a2 (Mir196a-2 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1-msfGFP
Plasmid#180328Purposemammalian expression of human SEPT9_i1 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD1-EGFP
Plasmid#131206Purposemammalian expression, tet RDepositorInsertNOD1 (NOD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i3-msfGFP
Plasmid#180322Purposemammalian expression of human SEPT9_i3 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v3 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-AsCpf1(RR)
Plasmid#109325PurposeAAV vector expressing AsCpf1 (RR variant)DepositorInserthAsCpf1(RR)
UseAAVTagsHA and NLSExpressionMutationS542R/K607RPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(Non-targeting)
Plasmid#109324PurposeAAV vector expressing non-targeting AsCpf1 crRNADepositorInsertmCherry-KASH
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i5-msfGFP
Plasmid#180327Purposemammalian expression of human SEPT9_i5 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v7 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + WT miR-181 BS in NLK 3UTR
Plasmid#22790DepositorInsertWild-type miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + MT miR-181 BS in NLK 3UTR
Plasmid#22791DepositorInsertMutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ERBB2_WT
Plasmid#82113PurposeGateway Donor vector containing ERBB2 , part of the Target Accelerator Plasmid Collection.DepositorInsertERBB2 (ERBB2 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ERBB2_WT
Plasmid#81892PurposeGateway Donor vector containing ERBB2 , part of the Target Accelerator Plasmid Collection.DepositorInsertERBB2 (ERBB2 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ERBB2_WT
Plasmid#82300PurposeGateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.DepositorInsertERBB2 (ERBB2 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only