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Plasmid#154008PurposeExpression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cellsDepositorInsertCodon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
UseTags3xFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
PCR4-Shh::lacZ-H11
Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterShhAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CDKN1B-Puro
Plasmid#172611PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cellsDepositorInsertCDKN1B (CDKN1B Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2 ORF3a C-V5-tag
Plasmid#179980PurposeMammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-2 ORF3a (ORF3a Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::lacZ-H11
Plasmid#139099PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHsp68Available sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 NSP3 C-V5-tag
Plasmid#179962PurposeMammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 Nsp3 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D
Plasmid#177320PurposeLentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).DepositorInsertKRAS 4B (KRAS Human)
UseLentiviralTagsFKBP-derived dimerization domain (DD, or DmrB) an…ExpressionMammalianMutationG12DPromoterTetOn-CMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry
Plasmid#172640PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-HA-NEK7
Plasmid#75142PurposeExpresses N-terminal HA-tagged NEK7 in mammalian cellsDepositorInsertNIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-Caspase-1
Plasmid#75128PurposeExpresses N-terminal Flag-tagged Caspase-1 in mammalian cellsDepositorInsertcaspase 1 (Casp1 Mouse)
UseTagsFlagExpressionMammalianMutationP341S in Caspase-1 (please see depositor comments…PromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
A*11:01:01
Plasmid#203281PurposeOverexpress HLA alleleDepositorInsertA*11:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
A*11:01:01:01
Plasmid#203276PurposeOverexpress HLA alleleDepositorInsertA*11:01:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS1089: mini-AAV.sgRNA.Nme2Cas9
Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianMutationPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCROPseq-VEX-optimized-scaffold
Plasmid#203321PurposeModified CROPseq vector for efficient CRISPR screens in hematopoiesisDepositorInsertsgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EmGFP-LATS2/1 KD
Plasmid#52085PurposeLentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporterDepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe.puro.CDK8.KD.flag
Plasmid#19759DepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseRetroviralTagsFlagExpressionMammalianMutationD173A (kinase dead)PromoterAvailable sinceDec. 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe.puro.CDK8.flag
Plasmid#19758DepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry
Plasmid#172620PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
B*58:02:01
Plasmid#203262PurposeOverexpress HLA alleleDepositorInsertB*58:02:01 (HLA-B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
A*66:01:01
Plasmid#203291PurposeOverexpress HLA alleleDepositorInsertA*66:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only