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Plasmid#218275PurposeIntroduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_BN006A-P_V5_FLAG_C
Plasmid#218062PurposeRESOLUTE SLC binder lentiviral plasmidDepositorInsertBN006A-P
UseLentiviralTagsV5-FLAGExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_BN001M-T_V5_FLAG_C
Plasmid#218063PurposeRESOLUTE SLC binder lentiviral plasmidDepositorInsertBN001M-T
UseLentiviralTagsV5-FLAGExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_BN006A-P_V5_mNG_FLAG_C
Plasmid#218076PurposeRESOLUTE SLC binder lentiviral plasmidDepositorInsertBN006A-P
UseLentiviralTagsFLAG-mNeonGreen-V5ExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN001M-T
Plasmid#218078PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN001M-T
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN001T-N
Plasmid#218077PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN001T-N
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN006A-P
Plasmid#218079PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN006A-P
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN001K-M
Plasmid#218084PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN001K-M
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN004M-4
Plasmid#218082PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN004M-4
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN008H-P
Plasmid#218081PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN008H-P
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN0059-C
Plasmid#218083PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN0059-C
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN006V-4
Plasmid#218080PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN006V-4
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-myr-AKT-HA
Plasmid#31789DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Synthetic, Human)
UseTagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFC902
Plasmid#106904PurposeThe vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1DepositorInsertscas9
pyrG
U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-Rbck1
Plasmid#63135PurposeExpression of human RBCK1/HOIL-1LDepositorInsertHOIL-1L (RBCK1 Synthetic, Human)
UseTagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-myr-AKT-HA
Plasmid#31790DepositorInsertAkt1 (Akt1 Mouse)
UseEntry vectorTagsHA and MyrExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/mEGFP-1T-IRES-mCherry
Plasmid#190190PurposeLentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.DepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationchanged EGFP Kozak sequence inserting a C>T mo…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWR63(YUM1)
Plasmid#203336Purposeepisomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR63
Plasmid#203331Purposeepisomal Cas9 and sgRNA expression vector without sgRNA for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only