-
-
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
UseTags6X Histidine and TEV cleavage siteExpressionYeastMutationPromoterADH1/yeastAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Golgi-targeted optoPLD
Plasmid#140058PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expressionDepositorInsertPhospholipase D
UseTagsCIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES_iSeroSnFR_cytosolic
Plasmid#214132PurposeFluorescent biosensor for intracellular serotonin (5-HT)DepositorInsertiSeroSnFR_cytosolic
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-TREX1
Plasmid#164245Purposeretroviral plasmid for GFP-TREX1DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G01
Plasmid#67135PurposeGateway destination vector for cell-free expression with N-term 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP-RPA70
Plasmid#164231Purposeretroviral plasmid for GFP-RPA70DepositorInsertRPA70 (RPA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyTagsExpressionMutationG65S, L68V, A72S, Y203T, L231HPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G04
Plasmid#67138PurposeGateway destination vector for cell-free expression with C-term eGFP 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HISExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
Golgi-targeted optodPLD
Plasmid#140057PurposeEncodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expressionDepositorInsertPhospholipase D
UseTagsCIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…ExpressionMammalianMutationH170A (catalytic dead)PromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)
Plasmid#173453PurposeContains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-TREX1 g2 Cas9-T2A-mCherry
Plasmid#164251PurposeTranscription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgTREX1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
Endosome-targeted optodPLD
Plasmid#140055PurposeEncodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expressionDepositorInsertPhospholipase D
UseTags2xFYVE, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationH170A (catalytic dead)PromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPS_puro
Plasmid#128504PurposeEncodes puromycin resistance proteinDepositorInsertPuromycin Resistance
UseLentiviralTagsExpressionMammalianMutationPromoterCyclophilin A promoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)
Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)
Plasmid#173454PurposeContains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-TREX1 g1 Cas9-T2A-mCherry
Plasmid#164250PurposeTranscription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgTREX1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ 3xFLAG-TREX1
Plasmid#164243Purposeretroviral plasmid for 3xFLAG-TREX1DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only
