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Showing: 581 - 600 of 52559 results

  1. pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

    Plasmid
    #201188
    Purpose
    Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    PTPN2, transcript variant 3 (PTPN2 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201188-plasmid-map-sequence-id-398098

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  4. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

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  6. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196878
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-N-gTBD
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196878-plasmid-map-sequence-id-408688

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  7. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

    Plasmid
    #196879
    Purpose
    Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196879-plasmid-map-sequence-id-408686

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  8. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

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  9. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196881
    Purpose
    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196881-plasmid-map-sequence-id-391885

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  10. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid
    #67275
    Purpose
    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do
    Insert
    puromycin resistant gene
    Use
    Tags
    linked to red flouresent protein gene (katushka)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67275-plasmid-map-sequence-id-118496

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Showing: 581 - 600 of 52559 results