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We narrowed to 35,147 results for: lat

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pcDNA 3.1 (+) FLAG-Ago2 S387E

Plasmid

#60653

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mammalian

Mutation

S387E mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387A

Plasmid

#60652

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mutation

S387A mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 5A

Plasmid

#60651

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated five serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLEX307_CR-PDHA2S291D

Plasmid

#115200

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_CR-PDHA2S293D

Plasmid

#115201

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_CR-PDHA2S291D/S293D

Plasmid

#115202

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 4A

Plasmid

#59907

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated four serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40R

Plasmid

#105303

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K40R

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40A

Plasmid

#105304

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K40A

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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ZE33 Prickle-1-GFPnopren-pCS2+

Plasmid

#17074

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mutation

Pk1GFPnopren deletes the C-terminal six amino aci…

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA6-mtIF3-Dendra2

Plasmid

#182371

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Use

Tags

Pal-Dendra2

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40Q

Plasmid

#105302

Purpose

To study effects of acetyl-mimetic (hyper-acetylated) tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K40Q

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)

Plasmid

#182370

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Use

Tags

Pal-Dendra2

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-pchD WT

Plasmid

#187973

Purpose

Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

Insert

pchD WT

Use

Tags

Hexahistag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)

Plasmid

#182369

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Use

Tags

Pal-Dendra2

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg2

Plasmid

#218531

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge