Addgene

We narrowed to 51,975 results for: cloning

Showing: 581 - 600 of 51975 results

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YIp LYS2-KanMx

Plasmid

#122920

Purpose

LYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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p28BIOHTEV-LIC

Plasmid

#167852

Purpose

Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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R777-E175 Hs.PIK3R3

Plasmid

#70459

Purpose

Gateway ORF clone of human PIK3R3 [NM_003629.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E159 Hs.PDPK1

Plasmid

#70443

Purpose

Gateway ORF clone of human PDPK1 [NM_002613.4] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

PDPK1 (PDPK1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E287 Hs.ROS1

Plasmid

#70571

Purpose

Gateway ORF clone of human ROS1 [NM_002944.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ROS1 (ROS1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E330 Hs.SPRY2-nostop

Plasmid

#70614

Purpose

Gateway ORF clone of human SPRY2 [NM_005842.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

SPRY2 (SPRY2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-H1-Esp3I-mU6-Kan

Plasmid

#213015

Purpose

Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)

Depositor

Stephanie Roessler

Insert

H1 promoter, mU6 promoter, gRNA scaffolds

Use

Tags

Expression

Mutation

Promoter

H1 promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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R777-E332 Hs.SPRY3-nostop

Plasmid

#70616

Purpose

Gateway ORF clone of human SPRY3 [NM_005840.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

SPRY3 (SPRY3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E221 Hs.RALGDS

Plasmid

#70505

Purpose

Gateway ORF clone of human RALGDS [NM_006266.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RALGDS (RALGDS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E348 Hs.TIAM1-nostop

Plasmid

#70632

Purpose

Gateway ORF clone of human TIAM1 [NM_003253.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

TIAM1 (TIAM1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E279 Hs.RHOB

Plasmid

#70563

Purpose

Gateway ORF clone of human RHOB [NM_004040.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RHOB (RHOB Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a CHR2 mCherry WPRE

Plasmid

#129432

Purpose

expression of CHR2 mCherry from EF1a promoter

Depositor

Oskar Laur

Insert

CHR2 mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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R777-E324 Hs.SPRED2-nostop

Plasmid

#70608

Purpose

Gateway ORF clone of human SPRED2 [NM_181784.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

SPRED2 (SPRED2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E356 Hs.TSC2-nostop

Plasmid

#70640

Purpose

Gateway ORF clone of human TSC2 [NM_001114382.1] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

TSC2 (TSC2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-UBC-DSRED-LUC-EF1 Hygro

Plasmid

#129437

Purpose

express dsred-luc chimera

Depositor

Oskar Laur

Insert

dsred-luc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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R777-E236 Hs.RASAL2-nostop

Plasmid

#70520

Purpose

Gateway ORF clone of human RASAL2 [NM_170692.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

RASAL2 (RASAL2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E325 Hs.SPRED3

Plasmid

#70609

Purpose

Gateway ORF clone of human SPRED3 [NM_001042522.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

SPRED3 (SPRED3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E165 Hs.PIK3CB

Plasmid

#70449

Purpose

Gateway ORF clone of human PIK3CB [NM_006219.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E281 Hs.RHOC

Plasmid

#70565

Purpose

Gateway ORF clone of human RHOC [NM_175744.4] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RHOC (RHOC Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E185 Hs.PLXNB1

Plasmid

#70469

Purpose

Gateway ORF clone of human PLXNB1 [NM_001130082.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

PLXNB1 (PLXNB1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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