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Plasmid#91088PurposeModule C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterOsU3Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EYFP
Plasmid#201792PurposeExpresses EYFP from a GAL promoter with a URA3 markerDepositorInsertEYFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-L3L2
Plasmid#114019PurposeCreate Entry clones for Gateway LR reactions through simple restriction enzyme based cloningDepositorInsertccdB gene, Chloramphenicol resistance gene
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-L1L4
Plasmid#114018PurposeCreate Entry clones for Gateway LR reactions through simple restriction enzyme based cloningDepositorInsertccdB gene, Chloramphenicol resistance gene
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-L1L2
Plasmid#114017PurposeCreate Entry clones for Gateway LR reactions through simple restriction enzyme based cloningDepositorInsertccdB gene, Chloramphenicol resistance gene
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pANY4
Plasmid#164897PurposeTwo valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-HIV-1_PR
Plasmid#203493PurposeExpresses HIV-1 protease from a GALL promoter with a URA3 markerDepositorInsertHIV-1 protease
UseTagsExpressionYeastMutationPromoterGALLAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
C79m
Plasmid#120991PurposeMoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdA antitoxin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-emiRFP703-stop
Plasmid#186386PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-emiRFP703-RfA
Plasmid#186376PurposeDestination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mVenus-stop
Plasmid#186394PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-miRFP713-RfA
Plasmid#186381PurposeDestination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mNeonGreen-stop
Plasmid#186392PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-miRFP713-stop
Plasmid#186391PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mEos4b-stop
Plasmid#186390PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mEos4b into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mClover3-stop
Plasmid#186389PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Rfa-mCherry-stop
Plasmid#186388PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mCherry into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mVenus-RfA
Plasmid#186384PurposeDestination vector for the expression of a fluorescent fusion of N-ter mVenus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mNeonGreen-RfA
Plasmid#186382PurposeDestination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mEos4b-RfA
Plasmid#186380PurposeDestination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only