Addgene

We narrowed to 1,049 results for: Anti-CRISPR

Showing: 581 - 600 of 1049 results

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pCMV_BE4max_3xHA

Plasmid

#112096

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1pr_v1

Plasmid

#97081

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1pr_v2

Plasmid

#97082

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax_3xHA

Plasmid

#112098

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max_3xHA

Plasmid

#112097

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1m_v1

Plasmid

#97083

Purpose

Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1m_v1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v1

Plasmid

#97085

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX335-NEAT1_IS_v2

Plasmid

#97086

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_355

Plasmid

#187159

Purpose

Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

Depositor

John Doench, David Root

Article

Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-10xGCN4_Hygro

Plasmid

#192651

Purpose

3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-10xGCN4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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L2_2xNOP1gRNA-Cas9-CsA

Plasmid

#136140

Purpose

Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

Plasmid

#155305

Purpose

Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d

Use

Lentiviral

Tags

2xNLS-3xFLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

Plasmid

#155307

Purpose

Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWBhis03

Plasmid

#188556

Purpose

Binary vector with MpIGPDm as a selective marker in Marchantia polymorpha (igpd mutants)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

MpIGPDm

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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SunTagCACTA1g2-22aa-TET1cd

Plasmid

#106437

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_502_sgCD45

Plasmid

#158705

Purpose

CD45 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD45 (PTPRC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-TC9

Plasmid

#202088

Purpose

entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

Depositor

David Edgell

Article

Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

Insert

TevCas9 + sgRNA cassette

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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