Addgene

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pT7-V5-SBP-C1-Hs4EBP1-L75AV81A_Z

Plasmid

#148144

Purpose

Mammalian Expression of Hs4EBP1-L75AV81A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EBP1-L75AV81A (EIF4EBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EBP1-Y54AL59A_Z

Plasmid

#148145

Purpose

Mammalian Expression of Hs4EBP1-Y54AL59A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EBP1-Y54AL59A (EIF4EBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EBP1-YLLVtoAAAA_Z

Plasmid

#148146

Purpose

Mammalian Expression of Hs4EBP1-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EBP1-YLLVtoAAAA (EIF4EBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF

Plasmid

#148686

Purpose

Bacterial Expression of HsGIGYF2_35-105-E46AE47FN91A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AE

Plasmid

#148575

Purpose

Mammalian Expression of HsGIGYF1_1-177-YYMLtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_1-177-YYMLtoAAAA (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_52-234opt_AE

Plasmid

#148564

Purpose

Bacterial Expression of Hs4EHP_52-234opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt (EIF4E2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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shAPI5#2

Plasmid

#157666

Purpose

Tet inducible knockdown of API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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shAPI5#1

Plasmid

#157665

Purpose

Tet inducible knockdown of API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-API5

Plasmid

#157660

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SL545

Plasmid

#49953

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-11 (RHOXF2 Human)

Use

TALEN

Tags

Expression

Mutation

Promoter

EF1a

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
D-raf G621S

Plasmid

#39769

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

G621S

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf C295S

Plasmid

#39768

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

C295S

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf F290I

Plasmid

#39766

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

F290I

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+S620A

Plasmid

#39765

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

R217L and S620A

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+C295S

Plasmid

#39764

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

R217L and C295S

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pX33

Plasmid

#89632

Purpose

Allelic change mutagenesis

Depositor

Greg Cook

Article

Gebhard et al Microbiology. 2006 Nov;152(Pt 11):3453-6

Insert

xylE

Use

Allelic change mutagenesis

Tags

Expression

Mutation

Promoter

Pmyco

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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TVT7R(0,0)

Plasmid

#98631

Purpose

Vector for cloning infectious RNA virus genomes

Depositor

Karyn Johnson, Andrew Ball

Article

Johnson et al J Virol. 2000 Jun;74(11):5123-32.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMT GFP

Plasmid

#126657

Purpose

Inducible expression of GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 7, 2019

Availability

Academic Institutions and Nonprofits only

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371 pTol2-fli1ep:MTDest

Plasmid

#73490

Purpose

Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:6xMyc-attR1-attR2

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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