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Plasmid#118082PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2(C506S, L4A)
Plasmid#136580Purposemammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)DepositorInsertNRF2 (C506S, Leuzip mut) (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationThe C506S mutation (C513S in the actual sequence …PromoterAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1 gRNA
Plasmid#117052PurposeIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoterDepositorInsertanti-mRFP1 SpCas9 gRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1E
Plasmid#183144PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1EDepositorInsertRBS, mRFP1E
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
J23117-mRFP1-331Bb
Plasmid#78280PurposemRFP1 behind constitutive promoter J23117 in pSEVA331Bb backboneDepositorInsertJ23117-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlmRFP1
Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterJ23100Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
J23114-mRFP1-331Bb
Plasmid#78279PurposemRFP1 behind constitutive promoter J23114 in pSEVA331Bb backboneDepositorInsertJ23114-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal mRFP1
Plasmid#104025PurposePlasmid encoding 2ndVal mRFP1DepositorInsert2ndVal mRFP1
UseLow copyTagsExpressionBacterialMutationmRFP1 with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
J23110-mRFP1-331Bb
Plasmid#78277PurposemRFP1 behind constitutive promoter J23110 in pSEVA331Bb backboneDepositorInsertJ23110-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT MEK5-DD-mRFP1
Plasmid#47547PurposeGateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutantDepositorInsertMEK5-DD (MAP2K5 Human)
UseEntry vectorTagsmRFPExpressionMutationS311D, T315DPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
J23112-mRFP1-331Bb
Plasmid#78278PurposemRFP1 behind constitutive promoter J23112 in pSEVA331Bb backboneDepositorInsertJ23112-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103-mRFP1-331Bb
Plasmid#78273PurposemRFP1 behind constitutive promoter J23103 in pSEVA331Bb backboneDepositorInsertJ23103-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23105-mRFP1-331Bb
Plasmid#78275PurposemRFP1 behind constitutive promoter J23105 in pSEVA331Bb backboneDepositorInsertJ23105-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23101-mRFP1-331Bb
Plasmid#78272PurposemRFP1 behind constitutive promoter J23101 in pSEVA331Bb backboneDepositorInsertJ23101-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Magenta
Plasmid#183140PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_MagentaDepositorInsertRBS, mRFP1E_Magenta
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Yellow
Plasmid#183141PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_YellowDepositorInsertRBS, mRFP1E_Yellow
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Orange
Plasmid#183143PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_OrangeDepositorInsertRBS, mRFP1E_Orange
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Violet
Plasmid#183139PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_VioletDepositorInsertRBS, mRFP1E_Violet
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Pink
Plasmid#183142PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_PinkDepositorInsertRBS, mRFP1E_Pink
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbAW4k-loxP-TT-loxP-mRFP1
Plasmid#134405PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.DepositorInsertW4-loxP-TT-loxP-mRFP1
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterW4 (modified T7A1)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only